盆底器官脱垂患者耻骨宫颈筋膜中TGF-β_1和CTGF的表达

目的:探讨盆底器官脱垂(POP)患者转化生长因子β1(TGF-β1)和结缔组织生长因子(CTGF)在耻骨宫颈筋膜组织中的表达。方法:选取30例盆底器官脱垂患者(POP组),其中Ⅱ级10例,Ⅲ级10例,Ⅳ级10例,采用免疫组化SP二步法检测耻骨宫颈筋膜中TGF-β1和CTGF的表达,并选择同期20例非卵巢功能性肿瘤和宫颈上皮内瘤变患者作为对照(对照组)。结果:TGF-β1和CTGF在POP患者耻骨宫颈筋膜组织中表达显著低于对照组(P<0.05),且其表达与POP-Q分期呈负相关(r=-0.409,-0.572;P<0.05);TGF-β1和CTGF两者的表达呈正相关性(r=0.401,P<0.05)。结论:POP患者盆底支持结构的退行性病变与组织中的TGF-β1和CTGF表达减少有关。

Expression of TGF-Beta_1 and CTGF in Pubocervical Fascia of Pelvic Organ Prolapse

Objective: To explore the relationship between the expression of transforming growth factor-β1(TGF-β1) or connective issue growth factor(CTGF) in pubocervical fascia and the occurrence of pelvic organ prolapse(POP).Methods: The biopsy specimens in pubocervical fascia were obtained from 50 subjects undergoing hysterectomia,who were assigned in the control,POP-QⅡ,POP-QⅢ and POP-QⅣ groups.The histological structures in pubocervical fascia were examined with routine HE staining and Masson's trichrome staining.TGF-β1 and CTGF in structures in pubocervical fascia were evaluated by immunohistochemical staining.Results: There were significant differences of TGF-β1 and CTGF between POP groups and control group(P<0.05).The expression of TGF-β1 and CTGF correlated with POP-Q(r=-0.409,-0.572;P<0.05).The expression of TGF-β1 in POP was also related with CTGF expression(r=0.401,P<0.05).Conclusion: There is a close relationship between degeneration of pelvic support construction and deficiency in TGF-β1 and CTGF expression in pubocervical fascia of POP patients.