制白附子70%乙醇提取物的完全急性毒性研究

目的：评价制白附子70%乙醇提取物的急性毒性作用。方法：灌胃给予大鼠25.0g/kg、50.0g/kg、100.0g/kg制白附子70%乙醇提取物1次,测定d1（给药当天）、d2、d3、d7、d14大鼠体重及给药前、给药后4h～28h、28～52h的饲料消耗量,计算摄食量,并于d3和d14各处死50%动物进行尸体解剖、血液生化学检查和血液细胞学检查。结果：给药第3d,低剂量组大鼠脾脏、胸腺系数增大（1.25±0.40,0.27±0.06）,高、低剂量组血清CREA含量下降（45.4±6.12μmol/L,41.7±21.4μmol/L）,高剂量组血清CK含量上升（2068.3±877.8IU/L）,以上数据与对照组比较均有统计学差异。给药14d,低、中剂量组血清CREA含量升高（70.6±15.6μmol/L,67.8±8.10μmol/L）。未观察到对大鼠体重、摄食量及血液细胞学指标有所影响。结论：制白附子70%乙醇提取物的三个受试剂量对大鼠急性毒性作用不明显。

Studying on the Completely Acute Toxicity of 70% Ethanolic Extract from Processed Rhizoma Typhonii on Rats

Objective：To investigate the toxic effect of processed Rhizoma Typhonii through completely acute toxicity experience.Methods：Administrated rats with 25.0g·kg-1、50.0g·kg-1、100.0g·kg-170% ethanolic extract of processed Rhizoma Typhonii once,respectively,then observed weight on day1、day 2、Day 3、day 7、day 14,feed consumption before and 4h-28h、28h-52h after administration.Those rats were killed 50% on day 3 and day 14.with autopsy,blood bio-chemical examination and hematological examination.Results：In the 3rd day of administration,speleen coefficient and thymus coefficient of 25.0g·kg-1 group increased（1.25±0.40,0.27±0.06）,compared with control.Serum CREA concentration decreased significantly in 25.0g·kg-1 and 100.0g·kg-1group（41.7±21.4μmol·L-1,45.4±6.12μmol·L-1,however,serum CK concentration increased significantly in 100.0g·kg-1 group（2068.3±877.8 IUL-1）.In the 14th day of administration,serum CREA concentration increased significantly in 25.0g·kg-1 and 50.0g·kg-1group（70.6±15.6μmol·L-1,67.8±8.10μmol·L-1）.There was little effect on rat＇s weight,feed consumption and hematological indexes.Conclusions：70% ethanolic extract of processed Rhizoma Typhonii in dosage of 25.0g·kg-1、50.0g·kg-1、100.0g·kg-1 had little effect on rats completely acute toxicity experience.