南沙参多糖对四氯化碳损伤原代培养大鼠肝细胞保护作用的研究

目的:探讨南沙参多糖(RAPS)对四氯化碳(CCl4)损伤原代培养大鼠肝细胞的保护作用及其机制。方法:通过原位灌流法分离大鼠肝细胞,培养36h后加入RAPS,同时造成CCl4损伤,分别于损伤24h和48h时检测培养液中丙二醛(MDA)的含量、丙氨酸氨基转移酶(ALT)、天冬氨酸氨基转移酶(AST)、超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-PX)的活性,48h后用MTT法测定肝细胞存活率。结果:RAPS对CCl4引起的ALT、AST活力的升高有抑制作用,同时抑制MDA的产生及肝细胞损伤造成的SOD活性及GSH-PX活性的降低。而且能明显改善肝细胞存活率。结论:RAPS能有效抑制CCl4造成的原代培养大鼠肝细胞损伤,机制可能与其抗氧化作用有关。

Protective Effects of Radix Adenophorae Potanini Korsh Polysaccharides on Carbon Tetrachloride-induced Injury of Primary Cultured Hepatocytes in Rats

OBJECTIVE:To study the protective effects and mechanism of Radix Adenophorae Potanini Korsh Polysaccharides(RAPS) on carbon tetrachloride(CCl4)-induced primary cultured hepatocytes injury in rats.METHODS:The rat hepatocytes were separated by perfusion in situ.RAPS was added to the cultured medium after incubating the rat hepatocytes for 36 hours,meanwhile,CCl4 was added to induce hepatocytes injury.The levels of ALT,AST,SOD,GSH-PX,and MDA after cultivation for 24h and 48h were determined respectively.And the survival rate of hepatocytes was determined at 48h by MTT method.RESULTS:RAPS could inhibit the CCl4-induced enhanced activities of ALT and AST,inhibit MDA production and the decreasing of SOD and GSH-PX induced by hepatocytes injury,and significantly improve the survival rate of hepatocytes.CONCLUSION:RAPS could effectively protect the primary cultured rat hepatocytes against CCl4 induced injury,which is possibly associated to its anti-oxidative activity.