温通针法对血管性痴呆模型大鼠血浆降钙素基因相关肽、内皮素含量的影响

目的:探讨温通针法对血管性痴呆(VD)大鼠的疗效机制。方法:健康Wistar大鼠50只,随机分为正常组、模型组、药物组、温通针法组和捻转针法组。采用反复夹闭双侧颈总动脉-再灌注方法造成VD大鼠模型,并给予相应的针刺("大椎"合谷"等)及药物(尼莫地平)治疗15d,采用放射免疫学方法检测大鼠血浆中降钙素基因相关肽(CGRP)及内皮素(ET)含量的变化。结果:VD模型组大鼠CGRP含量较正常组显著降低(P<0.01),ET含量较正常组显著升高(P<0.01);温通针法组、捻转针法组和药物组分别与模型组比较,均能明显升高CGRP含量(P<0.05,0.01),降低ET含量(P<0.01);温通针法组与药物组相比差异无显著性意义(P>0.05),且温通针法组升高CGRP含量、降低ET含量的作用优于捻转针法组(P<0.01)。结论:温通针法能使血浆中ET含量降低、CGRP含量升高,因而温通针法能有效改变机体中舒缩血管物质的含量,从而改善脑组织血液供应。

Effects of Warming-promotion Needling on Plasma Calcitonin Gene-related Peptide and Endothelin Contents in Rats with VD

Objective:To observe the effects of warming-promotion needling(WPN)on the levels of plasma calcitonin gene-related peptide(CGRP)and endothelin(ET)contents in vascular dementia(VD)rats so as to investigate its underlying mechanism in improving VD.Methods:A total of 50 Wistar rats were randomized into normal control,model,medication,WPN,and needle-twirling groups,with 10 cases in each.VD model was established by intraperitoneal injection of sodium nitroprusside(2.5 mg/kg)in combination with repeated occlusion of the bilateral common carotid arteries and reperfusion.For rats in medication group,Nimodipine(0.0108 g/kg)was given(oral perfusion),and for animals in WPN and needle-twirling groups "Dazhui"(GV 14),"Baihui"(GV 20)and "Shuigou"(GV 26)were punctured manually by manipulating the needle with WPN and common twirling methods respectively,60 s for each acupoint.The treatment was given once daily for 15 days.Plasma CGRP and ET contents were measured by radioimmunoassay.Results:Compared with normal control group,plasma CGRP content in model group decreased considerably(P<0.01),while plasma ET level in model group increased significantly(P<0.01).In comparison with model group,CGRP contents of WPN,needle-twirling and medication groups increased significantly(P<0.05,0.01),while plasma ET levels of WPN,needle-twirling and medication groups decreased significantly(P<0.01).The therapeutic effects of WPN group were significantly superior to those of needle-twirling group in raising CGRP content and lowering ET level(P<0.01);and the therapeutic effect of medication group was markedly superior to that of needle-twirling group in lowering ET content(P<0.01).No significant differences were found between WPN and medication groups in raising plasma CGRP and lowering ET level,and between needle-twirling and medication groups in raising CGRP content(P>0.05).Conclusion:Warming-promotion needling can effectively lower plasma ET and raise plasma CGRP level in VD rats,which may contribute to its clinical effect in improving VD patients' memory-learning ability.