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| 共固定化肿瘤坏死因子和干扰素的协同抗癌作用 | |
| 引用本文： | 关燕清,何丽梅,蔡树梅,周天鸿. 共固定化肿瘤坏死因子和干扰素的协同抗癌作用[J]. 生物医学工程学杂志, 2006, 23(6): 1303-1307 |
| 作者姓名： | 关燕清 何丽梅 蔡树梅 周天鸿 |
| 作者单位： | 1. 暨南大学,生命科学技术学院,广州,510632;华南师范大学,生命科学学院,生物技术研究所,广州,510631 2. 华南师范大学,生命科学学院,生物技术研究所,广州,510631 3. 暨南大学,生命科学技术学院,广州,510632 |
| 基金项目： | 广东省科技厅科技计划;中国博士后科学基金 |
| 摘 要： | 研究重组人干扰素（IFN-γ）和重组人肿瘤坏死因子（TNF-α）的共固定化对体外培养的人宫颈癌细胞（Hela）生长的影响。利用光固定化方法将IFN-γ、TNF-α共固定在24孔聚苯乙烯培养板（PSt）上，培养Hela细胞，分别设为：共固定（IFN-γ＋TNF-α）、单固定（IFN-γ、TNF-α）、游离（IFN-γ、TNF-α、IFN-γ＋TNF-α）各组培养，研究癌细胞生长抑制曲线，透射电镜、流式细胞仪检测Hela细胞的增殖和凋亡。结果表明共固定化干扰素和人肿瘤坏死因子对Hela细胞的抑制作用显著，最高达82％，显示IFN-γ对TNF-α具有良好的协同抗宫颈癌作用。 |
| 关 键 词： | 重组人干扰素(IFN-γ) 重组人肿瘤坏死因子(TNF-α) 协同作用 宫颈癌 光固定化 |
| 收稿时间： | 2004-10-18 |
| 修稿时间： | 2004-10-182005-02-03 |
Anti-cervix-cancer Effect of the Co-immobilization of Tumor Necrosis Factor-α and Interferon-γ |
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| Guan Yanqing,He Limei,Cai Shumei,Zhou Tianhong. Anti-cervix-cancer Effect of the Co-immobilization of Tumor Necrosis Factor-α and Interferon-γ[J]. Journal of biomedical engineering, 2006, 23(6): 1303-1307 | |
| Authors: | Guan Yanqing He Limei Cai Shumei Zhou Tianhong |
| Affiliation: | College of Life Science &Technology, Ji'an University, Guangzhou 510632, China. gyq69@163.com |
| Abstract: | To explore the effect of the co-photo-immobilization of Recombinant Human Interferon-r (IFN-gamma) and Recombinant Tumor Necrosis Factor (TNF-alpha) on the Hela cell cultivated in vitro. Co-immobilize the photoactive IFN-gamma and TNF-alpha on the polystyrene membrane; Cultivate the Hela cell on the cell culture polystyrene plate surface and set up the cultivation of the photo-immobilization IFN-gamma+TNF-alpha, Photo-immobilization IFN-gamma, nature (IFN-gamma, TNF-alpha, IFN-gamma+TNF-alpha). After 48 hours, the inhibition effect against Hela cells was studied in vitro by SEM and flow cytometry. It showed that the co-immobilization IFN-gamma+TNF-alpha has a more obvious inhibition effect on the Hela cell. And IFN-gamma can cooperate with TNF-alpha obviously. |
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