胆管癌组织螺杆菌16S-rRNA基因的检测及意义

目的探讨人胆管癌中是否存在螺杆菌的感染。方法取 11份手术切除的胆管癌标本 ,分别用两对螺杆菌属特异的 16S RNA引物进行PCR检测。对照组取自 4个肝囊肿和 2个血管瘤旁的正常肝胆管组织标本。结果 11份胆管癌标本用两对引物分别行二次PCR后 ,结果均为阳性的 6份 ,阳性率为 5 6 %。 4个肝囊肿和 2个肝血管瘤旁的正常肝胆管组织的PCR结果阴性 ,胆管癌组与对照组的阳性率有显著性差异 (P <0 0 5 )。对用C97和C98行PCR扩增的 2份产物测序 ,结果与幽门螺杆菌有 99%以上的同源性。结论胆管癌组织内存在螺杆菌的核酸物质 ,胆管癌患者肝脏中可能存在螺杆菌的感染。螺杆菌在胆管癌发生中的作用有待进一步研究。

Helicobacter-specific 16S ribosomal DNA identifided from patients with cholangiocarcinoma

Objective The aim of this study was to determine whether Helicobacter infection was associated with cholangiocarcinomas in human. Methods Resected liver tissues from 11 patients with cholangiocarcinoma were subjected to polymerase chain reaction(PCR) analysis using two sets of Helicobacter-specific 16S ribosomal RNA primers, and each sample was amplified twice. Control group included two cavernous hemangioma cases and four cyst cases. Results The expected 400-bp and 300-bp fragments of Helicobacter 16S rRNA were amplified from 6/11(55%) and 7/11(64%) cholangiocarcinoma samples, 6 samples were both positive for the two sets primers. The 6 control specimens were negative. The difference between the two groups was significant( P <0.05). In 2 patient samples from which 400-bp nucleotides were sequenced, the closest match was H. pylori. Conclusions These data suggest an association of bile-resistant Helicobacter spp. with cholangiocarcinomas. Further studies are needed to ascertain whether Helicobacter spp. infection plays a role in the development of cholangiocarcinomas.