兔胎盘来源间充质干细胞修复兔桡骨骨缺损的实验研究

目的 探讨兔胎盘来源间充质干细胞(PMSCs)构建的组织工程化骨修复兔桡骨节段性骨缺损的能力.方法 取24只大耳白兔,于兔双侧桡骨中下段制造1.5cm的节段性骨缺损,左侧骨缺损用PMSCs构建的组织工程化骨桥接(实验组),右侧用骨髓基质干细胞(BMSCs)构建的组织工程化骨桥接(对照组).实验动物双侧于术后2、4、8、...

Osteogenic capacity of placenta-derived mesenchymal stem cells in rabbits

Objective To compare osteogenic capacities of allogeneic bone marrow stromal cells (BMSCs)and planceta marrow stronal cells(PMSCs)to repair critical-sized segmental bone defects in rabbits.Methods Segmental osteoperiosteal defects of 1.5 cm were created surgically at bilateral radii in 24 rabbits.The defects at the left side were bridged with tissue engineered bone constructed by PMSCs (experimental group)while those at the right side were bridged with tissue engineered bone constructed by BMSCs(control group).The rabbits were observed generally after the operation.At 2,4,8,12 weeks postoperatively,X-rays of the bilateral radii were taken for radiographic assessments and the animals were sacrificed to harvest the involved radii.After macroscopic examination,the defects and their adjacent bone were prepared for histological and scanning electron microscopy observations as well as for biomechanical tests.Results The radiographic scores at 2,4,8,12 weeks postoperatively for the experimental and control groups were respectively 3.72 ±0.24 versus 3.49 ±0.29,6.24 ±0.41 versus 5.91 ±0.45,8.15 ±O.67 versus 8.87 ± O.59,and 11.39 ± 0.86 versus 12.04 ± 0.93,with no significant differences(P ＞0.05).Both intramembranous and endochondral ossifications were involved in the osteogenic processes of the bone defect repair,but the latter doninated the process.The maximum loads at 2,4,8,12 weeks postoperatively for the experimental and control groups were respectively 48.76 ± 6.03 N versus 56.17 ±4.10 N,65.64±4.49 N versus 74.67±7.69 N,101.04±3.35 N versus 90.77±6.91 N,and 131.35±6.91 N versus 150.61 ± 21.41 N,with no significant differences(P ＞ 0.05).Conclusions Tissue engineered bone constructed with either allogeneic PMSCs or BMSCs is capable of healing critical-sized segmental bone defects in rabbits.Since PMSCs have similar biological characteristics and osteogenic capacity compared with BMSCs,they can be used as a new source of seeding cells in bone tissue engineering.