In vivo labeling with1251-CRH of human ACTH-producing pituitary adenomas heterotransplanted to nude mice |
| |
Authors: | Uirich J Knappe Dieter K Lüdecke Maximilian J A Puchner Wolfgang Saeger Hans-D Herrmann |
| |
Institution: | 1. Department of Neurosurgery, University of Hamburg, Hamburg, Germany 3. Department of Pathology, Marienkrankenhaus, Hamburg, Germany
|
| |
Abstract: | Tissue from 23 pituitary adenomas causing Cushing’s disease was implanted subcutaneously into 159 NuNu/NMRi mice, resected
after 21 or 35 days, and evaluated histologically and immunohistochemically. After 21 days, 74.3% of the grafts survived,
59% having less than 30% necrotic adenoma cells. After 35 days, 45% of the adenoma fragments survived, 37% having less than
30% necrotic adenoma cells. The preservation of the grafts was essentially dependent on the grade of vascularization accomplished
by migration of the host’s capillaries. As assessed by adrenal weight and histologically, biological activity of the transplants
could not be detected. Histologically, the grafts maintained the features of their primary tumors, and adrenocorticotropic
hormone (ACTH) could be visualized immunohistologically.Seventeen mice with subsequently proved preserved adenoma tissue received
an intravenous injection of 12.5 μCi125l-corticotropin-releasing hormone (CRH) and light microscopy-autoradiography was performed. Specific labeling, as verified
by positive and negative controls, was exhibited by 1 1 of 15 transplants originating from 3 highly differentiated ACTH cell
adenomas. Four did not label clearly positive. Two grafts of an undifferentiated mucoid cell pituitary adenoma did not show
any labeling.The nude mouse model is a useful tool for the study of ACTH-producing pituitary adenomas in vivo. Highly differentiated
ACTH cell adenomas can be labeled with radioactive CRH in vivo. |
| |
Keywords: | |
本文献已被 SpringerLink 等数据库收录! |
|