脾气虚胃溃疡证病结合模型甲状腺组织乳酸脱氢酶和琥珀酸脱氢酶变化研究

目的 探讨脾气虚胃溃疡证病结合模型甲状腺组织代谢酶的改变，进一步揭示脾气虚胃溃疡和甲状腺之间的关系。方法 本实验是将大鼠制成脾气虚胃溃疡证病结合模型，对其进行加味四君子汤治疗，在溃疡最典型的第4天和溃疡基本愈合的第14天取材，进而将实验动物分第4天、第14天的脾溃组、治疗组、自然恢复组、盐水组各4组，对各组甲状腺组织的酶进行酶组化染色，通过图像分析其光密度值并经统计学处理。结果 与盐水组比较，脾溃组酶的活性降低，治疗组明显恢复，自然恢复组接近脾溃组。结论 脾气虚胃溃疡证病结合模型甲状腺出现了损伤，加味四君子汤对损伤的甲状腺有治疗作用。

To Probe the Changes of Enzymes in the Model of Splennic Asthenia Complicating Gastric Ulcer

Objective To probe the changes of enzymes in the model of splennic asthenia complicating gastric ulcer,and reveal the relation between thyroid gland and splennic asthenia gastric ulcer.Methods Building model of splennic asthenia complicating gastric ulcer in rats,treatment them in plus sijunzi decoction,selecting rats with typical gastric ulcer four days after and rats with the ulcer healing basi- cally fourteen days after building models,then the rats were divided into four groups,the model group,the treatment group,spontaneous re- covery group and the control group.With histochemical stain,the OD values of enzymes were analyzed with image analysis,and were ana- lyzed statistically.Results the activities of lactate dehydrogenase(LDH),succinate dehydrogenase(SDH),decreased.Compare the treat- ment group recovered.Conclusions In the model of splennic asthenia complicating gastric ulcer,some injury can occur on thyroid gland. plus sijunzi decoction has some treating action.