超声体外诱导白血病细胞K562凋亡的研究

 目的 研究超声对诱导白血病细胞K562凋亡的作用，为超声治疗的临床应用提供实验依据。方法 以人慢性粒细胞白血病急变细胞株K562为研究对象，用频率为1.8 MHz的超声波辐照白血病细胞K562，用光学显微镜观察凋亡细胞形态学改变，流式细胞仪检测细胞凋亡率。结果 超声辐照K562细胞后24 h，8 mV×5 min组及8 mV×10 min组细胞凋亡率分别为2.3 %和8.28 %；10 mV×5 min及10 mV×10 min组细胞凋亡率分别为9.36 %和21.46 %。电压为7mV时，超声辐照K562细胞后0.5 h，1 h，3 h，5 h细胞凋亡率分别为1.9 %，3.6 %，5.7 %，7.7 %。同时光学显微镜下可见凋亡小体形成等典型形态学改变。结论 超声可以诱导白血病K562细胞凋亡。

Study on ultrasound induced apoptosis of leukemia cell K562 cultured in vitro

Objective To observe the effect of ultrasound on apoptosis of K562 cells. Methods Human leukemia cell line( K562) cultured in vitro are treated by ultrasound at a frequency of 1.8 MHz. Apoptosis cells were evaluated by light microscope and detected using the flow cytometry assay（FCM）. Results The percentage of apoptosis in cells exposure to 8 mV×5 min and 8 mV×10 min were 1.1 % and 2.6 % respectively, 10 mV×5 min and 10 mV ×10 min were 9.7 % and 21.6 % respectively. The percentage of apoptosis in cells exposure to 7 mV×30 sec at 0.5 h, 1 h, 3 h, 5 h posttreatment were 1.9 %, 3.6 %, 5.7 %, 7.7 % respectively; Typical morphological changes of apoptosis such as apoptotic body were found under light microscope. Conclusion Ultrasound can induce apoptosis in vitro in human leukemia cell line K562