多次缺血预处理对兔脊髓缺血性损伤后脊髓功能和形态学的影响

目的观察多次缺血预处理(IPC)对兔脊髓缺血再灌注损伤的保护作用。方法24只日本大白兔随机分为假手术组(A组)、缺血再灌注组(B组)和IPC保护组(C组),各组均为8只。A组不阻断主动脉,B组左肾下阻断主动脉45min,C组左肾下阻断主动脉5min,开放5min,反复4次后再阻断主动脉45min。术后进行后肢神经功能评分和针电极肌电图(EMG)的描记及脊髓组织形态学改变的观察。结果B组同A、C组相比,后肢EMG亦有显著性病理改变(P<0.01)。结论多次IPC对家兔脊髓缺血再灌注损伤具有快速保护作用。

Protective effect of multi ischemic preconditioning on spinal cord ischemic and reperfusion injury in rabbit

Objective To observe the protective effect of multi ischemic preconditioning (IPC) on spinal cord ischemic and reperfusion injury in rabbit.Methods Twenty four rabbits were divided into sham operated group (group A, n=8), ischemic and reperfusion group (group B,n=8) and IPC group (group C,n=8). In group A, abdominal aorta wasn't crossclamped. Spinal cord ischemic was induced by 45 min of infra renal aortic occlusion in group B. Before the 45 min ischemic the rabbits in the IPC group underwent four cycles of ischemic preconditioning, i. e. occluding abdominal aorta for 5 min then reperfusing for 5 min. Postoperative neurologic function and EMG of rear limb, and spinal cord histopathological changes were assessed in all groups.Results Compared with group A and C respectively,the neurologic function and histopatholohical changes were more serious in group B (P< 0.01). And there was significantly worse change of EMG in group B than in group C (P< 0.01).Conclusion Multi ischemic preconditioning can protect rabbit spinal cord from ischemic reperfusion injury quickly.