复方丹参滴丸干预大鼠心肌再灌注损伤的机制研究

目的 从整体和细胞水平探讨复方丹参滴丸干预大鼠心肌再灌注损伤的作用机制。方法 以结扎大鼠冠状动脉左前降支（LAD）后松结造成急性心肌再灌注损伤（MRI）模型，在体外以培养心肌细胞缺氧乏糖后复氧复糖为模拟条件，观察复方丹参滴丸对MRI模型大鼠的心电图（EKG）、心肌酶[乳酸脱氢酶（LDH）、肌酸激酶（CK）]漏出量、心肌细胞内游离钙浓度、超氧化物歧化酶（SOD）和丙二醛（MDA）含量、内皮素-1（ET-1）和一氧化氮（NO）含量的影响。结果 复方丹参滴丸在整体和细胞水平均能改善MRI时大鼠EKG（P〈0．01）、减少心肌酶漏出量（P〈0.01）；降低复氧复糖心肌细胞内游离钙荧光强度、提高SOD活性（P〈0.01）减少MDA生成减少ET-1含量（P〈0.01），并适当增加NO含量（P〈0.01）。结论 复方丹参滴丸可能是通过作用于钙超载、氧自由基生成和内皮细胞损伤等环节有效干预MRI。

Studies on Mechanisms of the Effects of Fufangdanshen Bill on Acute Myocardial Reperfusion Injury in Rats

Objective To study the Mechanisms of the effects of Fufang damshen Bill(FDS) on acute myocardial reperfusion injury (MRI) in vivo and in vitro.Methods The left anterior descending coronary artery (LAD) of the rat was ligated for 30 minuts and the ligature was released to make the model of reperfution.Neonatal rat cardiomyocytes were cultured in the medium with six hours hypocia followed two hours reoxygenation as the equal condition in vitro.Results FDS could improve the electrocardiogram(P<0.01),reduce the leakage of Lactate dehydrogenase (LDH )(P<0.01)and creatine kinase (CK)(P<0.01)of rats with MRI.FDS could reduce the FI of calcium image cardiomyocyte,increase the activity of SOD(P<0.01)and decrease the production of MDA(P<0.01),ecrase the content of ET-1(P<0.01) and increase the content of NO(P<0.01) of rats with MRI.Conclusion FDS could protect the myocardial cells from reperfusion injury by reducing calcium overloading,scanvering OFR and maintaining the balance of ET/NO.