大鼠胰岛与生长因子共微囊提高囊内胰岛存活率

目的 探讨微胶囊胰岛皮下移植治疗化学性糖尿病小鼠和生长因子(growth factor,GF)与大鼠胰岛共微囊对提高囊内胰岛存活率的作用.方法 选取C57BL/6雄性小鼠皮下预血管化糖尿病模型25只,行微胶囊大鼠胰岛皮下移植.随机分为5组,每组5只.①A组:GF与大鼠胰岛共微囊移植组;②B组:微胶囊大鼠胰岛移植组;③C组:GF微囊移植组;④D组:大鼠胰岛移植组;⑤E组:空囊移植组.观察受体小鼠的血糖和体重变化,移植后8周比较微胶囊内胰岛存活数.结果 移植后1周至移植后3周A组与B组间血糖均有非常显著差异(P＜0.01),移植后4周至移植后8周A组与B组间血糖无显著差异(P＞0.05),移植后1周至移植后4周B组与C组间血糖均有非常显著差异(P＜0.01).C组血糖异常升高,移植4周后小鼠陆续死亡.D组和E组移植无效.A组和B组间各实验点小鼠体重均无显著差异(P＞0.05),A组小鼠体重成模时下降,移植2周后开始上升.A组和B组胰岛存活数有非常显著差异(F=36.84,P＜0.01).结论 GF与大鼠胰岛共微胶囊移植,GF可促进胰岛的细胞增殖和再生,显著提高囊内胰岛的存活率.

Growth factor coencapsulated with rat islets improves survival of encapsulated islets

Objective To see whether prevascularized subcutaneous transplantation of SD rat islets coencapsulated with growth factor (GF) normalizes blood glucose of STZ-induced diabetic mice and improves survival of encapsulated SD rats islets. Methods Twenty five subcutaneously prevascularized streptozocin(STZ)- induced diabetic mice using microencapsulated islets for transplantation were equally randomized to 5 groups: Group A, using SD rat islets coencapsulated with GF for prevascularized subcutaneous transplantation; Group B, using encapsulated rat islets for prevascularization subcutaneous transplantation; Group C, D and E, serving as controls. Post- transplantation blood glucose and body weight of STZ- induced diabetic mice were observed. The number of surviving rat islets was counted 8 weeks after transplantation. Results The mean blood glucose level of Group A and B was normalized 7 days after transplantation. Xenotransplantation reversed the loss of body weight and led to a continuous increase in body weight, the difference between the two groups being insignificant. DTZ- positive microencapsules were counted 8 weeks after transplantation. More DTZ- positive microencapsules were found in Group A (F=36.84, P<0.01). Conclusions Rat islets coencapsulated with GF prevascularization subcutaneous transplantation is an effective strategy for promoting proliferation and regeneration of islets and improves survival of encapsulated islets of diabetic animals.