Opposite Effects of 5-Hydroxymethyluracil on Mitogenic Response of T Cells Stimulated through T-Cell Receptor or through T-Cell Receptor and CD28 Co-Receptor Molecule |
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Authors: | S Sh Mananova I A Pashnina S V Sibiryak |
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Institution: | Institute of Immunology and Physiology, Ural Division of the Russian Academy of Sciences, Ekaterinburg, Russia. |
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Abstract: | The effect of a synthetic analog of “minor” pyrimidine base 5-hydroxymethyluracil (0.01- 10 μg/ml) on the mitogenic response
of donor peripheral blood T cells was studied in vitro under conditions of lymphocyte stimulation through T-cell receptor (antiCD3 monoclonal antibodies) or through T-cell receptor
and CD28 co-receptor molecule (antiCD3 and antiCD28 monoclonal antibodies). 5-Hydroxymethyluracil suppressed the mitogenic
response of T cells stimulated with antiCD3 antibodies, which was paralleled by an increase in the count of silent cells and
decrease in the count of dividing lymphocytes, but not by stimulation of apoptosis of activated cells. Under conditions of
integration of stimuli (lymphocyte stimulation with antiCD3 and antiCD28), 5-hydroxymethyluracil stimulated the mitogenic
response, which was paralleled by suppression of activation apoptosis and increase in proliferative potential of cells. |
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