| 陈皮总DNA提取方法的研究 |
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| 引用本文： | 严寒静,高晓霞,陈晓颖. 陈皮总DNA提取方法的研究[J]. 广东药学院学报, 2004, 20(6): 592-593 |
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| 作者姓名： | 严寒静 高晓霞 陈晓颖 |
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| 作者单位： | 广东药学院,药用植物与生药学教研室,广东,广州,510240;广东药学院,药物分析化学教研室,广东,广州,510240 |
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| 摘 要： | 目的寻找一种以市售中药材陈皮为材料，可获得高质量的陈皮总DNA的方法。方法采用CTAB法提取总DNA，在研磨过程加入PVP，经琼脂糖凝胶电泳检测。结果用非可溶性PVP(Polyvinylpyrrolidone)研磨，CTAB法能较好的去除色素、多糖等干扰物质。结论该方法可以用于多糖、色素含量高的中药材的总DNA的提取。
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| 关 键 词： | 陈皮 总DNA PVP |
| 文章编号： | 1006-8783(2004)06-0592-02 |
| 修稿时间： | 2004-05-11 |
Total DNA extraction from Pericarpium Citri Reticulatae |
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| YAN Han-jing ,GAO Xiao-xia ,CHENG Xiao-ying. Total DNA extraction from Pericarpium Citri Reticulatae[J]. Academic Journal of Guangdong College of Pharmacy, 2004, 20(6): 592-593 |
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| Authors: | YAN Han-jing GAO Xiao-xia CHENG Xiao-ying |
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| Affiliation: | YAN Han-jing 1,GAO Xiao-xia 2,CHENG Xiao-ying 2 |
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| Abstract: | Objective To explore the method for extraction of high quality DNA from Pericarpium citri Reticulatae. Methods Two methods, i.e., CTAB, and modified CTAB with PVP, were used to extract total DNA from traditional Chinese medicinal material Pericarpium citri Reticulatae. Results The second method was preferable in removing pigments, polysaccharides, which disturbing PCR. Conclusion This method can be used in extracting DNA from plant Chinese medicinal material that has high contents of pigments and polysaccharides. |
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| Keywords: | Pericarpium Citri Reticulatae total DNA PVP |
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