过敏性紫癜患儿血清 IL-17与 MMP-9的变化及意义

目的：观察过敏性紫癜患儿血清白细胞介素-17（interleukin-17，IL-17）、基质金属蛋白酶-9（ matrix metalloproteinase-9，MMP-9）的水平变化及其相关性，进一步探讨两者在过敏性紫癜及紫癜性肾炎中的发病机制。方法采用ELISA法检测74例过敏性紫癜初期患儿和30例健康体检儿童血清IL-17与MMP-9的水平。结果（1）过敏性紫癜组患儿血清 IL-17（86.59±35.50） fg/L ]高于健康对照组（62.38±14.65） fg/L]（P＜0.01）。（2）过敏性紫癜患儿血清MMP-9（201.82±105.87） pg/L]高于健康对照组（89.27±27.99） pg/L]（P＜0.01）。（3）密切随访过敏性紫癜组患儿6个月。6个月时，根据是否有肾脏受累分为非紫癜肾组和紫癜肾组，紫癜肾组在病初血清IL-17101.67±39.55] fg/L]高于非紫癜肾组（81.38±32.79） fg/L]（P＜0.05）；紫癜肾组在病初血清MMP-9（249.63±97.57） pg/L]高于非紫癜肾组（185.30±104.39） pg/L]（P＜0.05）。（4）过敏性紫癜组血清IL-17与MMP-9无相关性（r ＝0.184，P＞0.05）。结论 IL-17与MMP-9均参与过敏性紫癜、紫癜性肾炎的发病过程。

Changes and significance of serum interleukin-17 and matrix metalloproteinase-9 in children of henoch-schonlein purpura

Objective To investigate the chanegs and correla iton between IL-17 and MMP-9 in c hildren of henoch-schonlein purpura,and to explore the pathogenesis of teh IL-17 and MMP-9 in henoch-schonlein pur-pura /henoch-schonlein purpura nephritis.Methods The level of serum IL-17 andMMP-9 in 74 henoch-schon-lein purpura childr en in initial stage and 30 cases of healthy children（ control group） were detetced by ELISA. Results （1） The level of plasma IL-17 was significantly higher in children of henoch-schonlein purpura thna tha t foh ealthy control group （86.59 ±35.50） fg/L vs.（62.38 ±14.65）fg/L,P〈0.01].（2） The level of plasma MMP-9 was significantly higher in children of henoch-schonlein pupru ar than that o fhealt hy control group （201.82 ±105.87） fg/L vs（89.27 ±27.99）fg/L,P 〈0.01].（3） Henoch-schonlein purpura children were followed up for6 months,dependin g on whether the kidney was involved,they were devided into non-henoch-schonlein purpura nephritsi grou p nad henoch-s chonlein purpura nephritis group.In initial phase of HSP,the level of plasma IL-17 was significant lyhigher in henoch-schonlein purpura nephritis group than non-henoch-schonlein purpura nephrits group （101.67 ±39.55） fg/L vs.（101.67 ±39.55）fg/L,P〈0.05].The level of p lasma MMP-9 was significantly higher in henoch-schonlein purpura nephritsi group than no n-henoc h-schonlein purpura nephritis group （249.63 ±97.57） Pg/L vs.（185.30 ±104.39） pg/L,P〈0.05 ].（4） IL-17 and MMP-9 correlation aanlysis didn＇t show sign ificant correlation（r=0.184,P〉0.05）.Conclusion IL -17 and MMP-9 are involved in the pathogenesis of henohc-schonlein purpura/henoch-schonlein pur pura nephritis.