水通道蛋白5过表达与宫颈癌发生及临床病理特征的相关性

目的：研究水通道蛋白5（aquaporin 5，AQP5）在宫颈癌中的表达及临床病理意义，探讨AQP5在宫颈组织恶性转化过程中的可能作用。方法：采用蛋白质免疫印迹和免疫组织化学技术检测30例正常宫颈、28例宫颈上皮内瘤样变（CIN）和56例宫颈鳞状细胞癌组织中AQP5的表达，并对其表达的临床病理参数进行统计学分析。结果：AQP5在正常宫颈、CIN和宫颈癌组织中相对蛋白表达量逐渐升高，3组间差异有统计学意义（F=38.516，P=0.000）；其表达的平均阳性细胞百分比分别为15.12%、23.18%和36.08%，3组间差异有统计学意义（χ2=22.404，P=0.000）；AQP5过表达与宫颈癌淋巴结转移相关（r=0.351，P=0.008），与患者年龄、肿瘤大小、组织学分级和国际妇产科联盟（FIGO）分期无关。结论：增强的AQP5表达与正常宫颈组织恶性转化和宫颈癌发生有关，有望为宫颈癌提供一种新的治疗靶点和预后标记。

Correlation of AQP5 Up-regulation and Carcinogenesis of Cervical Carcinoma and Its Clinicopathological Characteristics

Objective：To investigate the expression of aquaporin 5 （AQP5） in cervical carcinoma and its clinicopathological significance，and the potential effect of AQP5 during the malignant transformation of normal cervical endotheliment. Methods：The expression of AQP5 were detected by western blotting and immunohistochemistry in 30 cases of normal cervical tissues，28 cases of cervical intraepithelial neoplasia （CIN） and 56 cases of cervical squamous cell carcinoma. The clinicopathological parameters of the protein were analyzed statistically. Results：The relative quantity of AQP5 in normal cervical endotheliment, CIN and cervical carcinomas increased gradually. There were significant difference between the 3 groups（F=38.516，P=0.000）. The mean percentage of positive cells were 15.12%,23.18% and 36.08% respectively. There were significant difference between the 3 groups( χ2=22.404，P=0.000). Overexpression of AQP5 was significantly correlated with lymph node metastasis （r=0.351，P=0.008）. No correlation was found between AQP5 protein expression and age，tumor size，histological grade or FIGO stage. Conclusions：These observations suggest that increased expression of AQP5 were related to the malignant transformation of normal cervical endotheliment and the carcinogenesis of cervical carcinoma and therefore may provide a novel therapeutic target as well as a prospective biomarker for cervical carcinoma.