| PEG6000沉淀结合差速离心方法浓缩水中脊髓灰质炎病毒 |
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| 引用本文： | 张文清,马文煜,骆文静,姜绍谆,胡艳冰,侯悦,张进,于碧云. PEG6000沉淀结合差速离心方法浓缩水中脊髓灰质炎病毒[J]. 医学争鸣, 2000, 21(1): 38-40 |
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| 作者姓名： | 张文清 马文煜 骆文静 姜绍谆 胡艳冰 侯悦 张进 于碧云 |
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| 作者单位： | 1. 第四军医大学,预防医学系军队卫生学教研室,陕西,西安,710033
2. 第四军医大学,基础部微生物学教研室,陕西,西安,710033 |
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| 摘 要： | 建立一种简便，有效的从细菌培养物中纯化ＰＶ１病毒的方法用于水病毒消毒实验。方法ＰＶ１Ｈｅｎａｎ株在Ｈｅｐ－２细胞中增殖后用聚乙二醇６０００沉淀结合差速离心的方法纯化病毒，ＲＴ－ＰＣＲ和透射电镜鉴定提纯病毒的形态学和分子生物学特性。结论ＰＥＧ６０００结合差速离心的方法较之蔗糖密度梯度区带离方法易于操作且比单独ＰＥＧ沉淀有更高的纯化系数，是一种简便，有效的方法。
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| 关 键 词： | 脊髓灰质炎病毒 纯化 聚乙二醇 水消毒 离心法 |
Establishment of cell infection model for inactivation of poliovirus in water |
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| ZHANG Wen-Qing,MA Wen-Yu,LUO Wen-Jing,JIANG Shao-Zhun,HU Yan-Bing,HOU Yue,ZHANG Jin,YU Bi-Yun. Establishment of cell infection model for inactivation of poliovirus in water[J]. Negative, 2000, 21(1): 38-40 |
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| Authors: | ZHANG Wen-Qing MA Wen-Yu LUO Wen-Jing JIANG Shao-Zhun HU Yan-Bing HOU Yue ZHANG Jin YU Bi-Yun |
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| Abstract: | AIM To establish a convenient and effective method for the isolation and purification of polioviruses. METHODS PV1 Henan strain propagated in Hep 2 cells was precipitated by PEG 6000 in combination with differential centrifugation. RESULTS 7% final concentration of PEG 6000 at a pH value of 7.5 was found to give a 79.2% recovery of virus infectivity and the purification factor (PF) was 106. The recovery and PF with differential centrifugation were 29.0% and 168. Transmission electron microscopy (TEM) and RT PCR determined viral morphologic and molecular biological characterization of the final preparation. CONCLUSION This method is easier to operate than sucrose density gradient rate zonal centrifugation and has a higher PF than that of PEG 6000precipitation alone. |
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| Keywords: | poliovirus isolation and purification PEG6000 centrifugation |
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