Ⅰ、Ⅱ相代谢酶基因多态性与肺癌易感性关系的研究

目的探讨Ⅰ相代谢酶CYPlA1、2E1、2D6和Ⅱ相GSTM1基因多态性与肺癌遗传易感性的关系及吸烟与基因之间的交互作用。方法 采用病例对照研究和聚合酶链反应-限制性片段长度多态性等技术,检测217例肺癌患者和200例对照CYPlA1、2E1、2D6和GSTM1基因型频率分布以及与吸烟的关系。结果 肺癌组GSTM1缺陷型频率为58.5％,与对照组(47.5％)比较差异有统计学意义(P=0.02);CYPlA1、2E1、2D6在肺癌组和对照组分布差异无统计学意义(P>0.05)。吸烟与GSTM1有协同作用,与CYPlA1、2E1、2D6未见明显的协同作用。结论 吸烟和GSTM1缺陷型均是肺癌的危险因素,GSTM1缺陷型有吸烟行为的人更易患肺癌,是肺癌的高危人群。

The relationship between genetic polymorphism of metabolizing enzymes and the genetic susceptibility to lung cancer

Objective To investigate the relationship between the gene. polymorphism of metabolizing enzymes and the genetic susceptibility to lung cancer as well as to study the synergistic effects between smoking and the genes. Methods A case-control study (case = 217,control = 200) was carried out to compare the frequent distribution of CYP1A1,2E1,2D6 and GSTMl genotypes between the lung cancer group and the control group with a polymerase chain reaction-restriction fragment polymorphism (PCR-RFLP) method and to analyze the relationship between these genes and smoking. Results GSTMl-null genotype frequency was 58.5% in the lung cancer group and 47.5% in the control group vvith significant difference( P = 0.02) . The frequent distribution of CYP1 A1, 2E1, 2D6 genotypes was not significantly different in the two groups(P>0. 05). Synergistic effects were found between smoking and GSTMl but not between smoking and CYP1A1,2E1,2D6. Conclusion Smoking and GSTMl-null genotype seemed to be the risk factors of lung cancer. Those who carrying GSTMl-null genotype and smoking cigarettes were prone to suffer from lung cancer to become the high-risk population of the disease.