HPLC法同时测定菊兰感冒颗粒中绿原酸、黄芩苷和木犀草素的含量

目的：建立同时测定菊兰感冒颗粒中绿原酸、黄芩苷和木犀草素等3种成分的HPLC方法。方法：采用Diamosil C18（2）色谱柱（250 mm×4.6 mm,5μm）;乙腈-0.2%磷酸溶液为流动相,流速为1.0 ml·min^-1,梯度洗脱;柱温35℃;检测波长为327 nm（绿原酸和黄芩苷）、360 nm（木犀草素）。结果：绿原酸、黄芩苷和木犀草素的线性范围分别为0.50-9.96μg·ml-1（r=0.999 8）、10.10-202.00μg·ml^-1（r=0.999 5）、0.10-2.04μg·ml^-1（r=0.999 6）;平均回收率分别为98.13%、98.80%、99.62%;RSD分别为0.65%、0.26%、0.57%（n=6）。结论：该法简便、稳定、重复性好,可用于菊兰感冒颗粒中绿原酸、黄芩苷和木犀草素的含量测定。

Simultaneous Determination of Chloroginic Acid, Baicalin and Luteolin in Julan Ganmao Particles

Objective：To establish an HPLC method for the simultaneous determination of chloroginic acid, baicalin and luteolin in Julan Ganmao particles. Methods：A Diamosil C18 （2） column（250 mm × 4. 6 mm,5μm） was used. The mobile phase consisted of acetonitrile and 0. 2% phosphoric acid with gradient elution. The flow rate was 1. 0 ml·min-1 , the column temperature was 35℃, and the detection wavelength was at 327 nm for chloroginic acid and baicalin, and 360 nm for luteolin. Results：Chloroginic acid, baicalin and luteolin was in a good linear relationship within the range of 0. 50-9. 96 μg·ml-1（r=0. 999 8）, 10. 10-202. 00 μg·ml-1 （r=0.999 5）, and 0.10-2.04 μg·ml-1（r=0.999 6） with the recovery of 98.13%（RSD=0.65%）, 98.80%（RSD=0.26%） and 99. 62%（RSD=0. 57%）, respectively. Conclusion： The developed method is simple, accurate and reliable with good repeatability, and can be used in the quality control of chloroginic acid, baicalin and luteolin in Julan Ganmao particles.