| 硒蛋白P同功型突变体短肽hSel P82m分析 |
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| 引用本文： | 王青,窦科峰,钱海利,马庆久,鲁建国,宁力,房青,林晨.硒蛋白P同功型突变体短肽hSel P82m分析[J].第四军医大学学报,2005,26(10):872-874. |
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| 作者姓名： | 王青 窦科峰 钱海利 马庆久 鲁建国 宁力 房青 林晨 |
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| 作者单位： | 第四军医大学西京医院肝胆外科,陕西,西安,710033;中国医学科学院中国协和医科大学肿瘤研究所分子肿瘤学国家重点实验室,北京,100021;第四军医大学唐都医院普通外科,陕西,西安,710038 |
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| 摘 要： | 目的: 切割硒蛋白P同功型突变体活性肽hSel P137m,以得到可能具备相同生物学活性而氨基酸数目更少的短肽.方法: 利用生物信息学技术分析hSel P280m,hSel P137m的活性功能域,用羟胺切割hSel P137 m,并用SDS-PAGE及蛋白质印迹加以分析验证.结果: 切割hSel P137m后得到了82(28aa-109 aa)个氨基酸的多肽,在特定的位置出现了预期的目的蛋白条带.利用生物信息学技术加以分析,认为hSel P82m基本具备hSel P137m的生物学活性.结论: 经切割硒蛋白P同功型突变体活性肽hSel P137m,成功得到82个氨基酸的短肽hSel P82m,并且认为其基本具备hSel P137m的生物学活性.
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| 关 键 词： | 硒 蛋白质类 肽类 计算生物学 化学裂解 印迹法 蛋白质 |
| 文章编号： | 1000-2790(2005)10-0872-03 |
| 修稿时间： | 2005年3月7日 |
Preparation and analysis of human selenoprotein P mutant peptide hSel P82m |
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| WANG Qing,DOU Ke-Feng,QIAN Hai-li,MA Qing-jiu,LU Jian-guo,NING Li,FANG Qing,LIN Chen.Preparation and analysis of human selenoprotein P mutant peptide hSel P82m[J].Journal of the Fourth Military Medical University,2005,26(10):872-874. |
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| Authors: | WANG Qing DOU Ke-Feng QIAN Hai-li MA Qing-jiu LU Jian-guo NING Li FANG Qing LIN Chen |
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| Abstract: | AIM: To obtain the potentially active shorter form of peptide with hSel P137m activity by chemically splitting SelP peptide mutant isoform.METHODS: The potential active domains of mutant hSel P280m and hSel P137m were analyzed by bioinformatics.hSel P137m was split by hydroxylamine and the result was analyzed by SDS-PAGE and Western blotting.RESULTS: The SDS-PAGE showed that hSel P137m was chemically split at the expected position between 28aa and 109aa.The split was also confirmed by Western blotting with polyclonal anti-hSel P137 antibody.Bioinformatics indicated that the hSel P82m fragment had similar biological activity to that of hSel P137m.CONCLUSION: The fragment hSel P82m has been obtained by cutting hSel P137m.Bioinformatical analysis indicates that the hSel P82m fragment has similar biological activity to that of hSel P137m. |
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| Keywords: | selenium proteins peptides computational biology chemically-splitting Blotting Western |
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