MUG－EMB快速检定大肠杆菌的方法研究

通过对不同来源的５９１株大肠杆菌的测试，其β－葡萄糖醛酸酶阳性反应占菌株总数９４．８％。与部颁法比较，对１２０个样品进行大肠杆菌检测，两法均检出大肠杆菌的有１０个样品，未检出大肠杆菌的有１０９个样品，符合率为９９．１％；对２０５株阳性对照菌在样品中检测，两法同时检出大肠杆菌１９９株，未检出６株，符合率为９７．１％。改进的ＭＵＧ－ＥＭＢ法与部颁法的准确度、灵敏性完全一致。革兰氏阳性杆菌、球菌、芽胞菌属一些菌株亦有β－葡萄糖醛酸苷酶。

Studies on MUG-EMB method for rapid detection of escheirchia coli

The positive rate was 94.8%in 591 E.coli strains obtained from different sources by testing β-glucuronidase with MUG method.In testing another 120 samples,the results of 10 E.coli and 109 non E.coli samples were the same,but only 1 sample was showing different between MUG method and the standard method issued by the Chinese Ministry of Public Health. The consistency was 99.1%. It indicated that MUG method was as good as the standard method in accuracy and sensitivity. The home-made MUG reagent used in these studies had the same quality standard as imported MUG reagent,so it provided a good condition for detecting E.coli and coliform in pharmaceutical products , food products and drinking water by this methed.