BAD和Bcl-X/L基因在小鼠胸腺细胞凋亡时的表达及其意义

目的检测BAD和Bcl-X/L基因在小鼠胸腺细胞凋亡的表达,探讨细胞凋亡调控基因在小鼠胸腺细胞凋亡时的作用。方法应用免疫组织化学染色法,观察不同剂量糖皮质激素诱导的小鼠胸腺细胞凋亡时BAD和Bcl-X/L基因的表达。结果免疫组织化学结果表明,正常对照组胸腺内BAD呈低表达,地塞米松组随剂量的增加BAD的表达成递增趋势,地塞米松各组与对照组比较差异有有统计学意义(P<0.05);正常对照组胸腺内Bcl-X/L呈高表达,地塞米松组随剂量的增加Bcl-X/L的表达成递减趋势,地塞米松各组与对照组比较差异有统计学意义(P<0.05)。结论促凋亡蛋白BAD和抗凋亡蛋白Bcl-X/L在糖皮质激素诱导引起的胸腺细胞凋亡调控中起重要作用。

Expression of BAD and Bcl-X/L gene expression in mice thymocyte apoptosis and its significance

Objective To detect the expression of BAD and Bcl-X/L gene in mice thymocyte apoptosis, and to investigate the effect of apoptosis regulated gene in the mice thymocyte apoptosis. Methods The expression of BAD and BCL-X/L gene in mice thymocyte apoptosis were induced by various dose of glucocorticoids, and the gene expression were determined by immunohistochemical staining. Results Immunohistochemical staining showed that BAD expression increased as the dose increased in the DEX-groups whereas the expression of BAD in the controls was low. Significant differences were found between the DEX-groups and the controls (P<0.05). The Bcl-X/L expression decreased as the dose increased in DEX-groups whereas in the controls BAD were overexpression. Significant differences were found between the DEX-groups and the controls (P<0.05). Conclusion BAD and Bcl-X/L played an important role in the glucocorticoids-induced thymocyte apoptosis.