正常与异位子宫内膜Bcl-2表达与细胞凋亡的关系

目的 :探讨 Bcl- 2和细胞凋亡与在位及异位子宫内膜的关系。方法 :应用免疫组化法对61例正常月经周期子宫内膜及卵巢子宫内膜异位症患者进行 Bcl- 2检测。同时用 TUNEL法对其中 34例标本进行凋亡检测。结果 :正常位置的子宫内膜分泌期及月经期凋亡细胞较多 ,增生期内膜及异位内膜无凋亡。 Bcl- 2蛋白在增生期含量高 ,分泌期低落 ,月经期消失 ,异位内膜中高于增生期( P<0 .0 5 )。结论 :细胞凋亡与子宫内膜脱落有关 ;异位子宫内膜细胞通过抗凋亡基因 Bcl- 2的高表达而抑制细胞的凋亡过程 ,延长生命周期

The Relationship Between Expression of Bcl-2 and Cell Apoptosis in Eutopic and Ectopic Human Endometrium

Objective: To study the relationship between bcl 2 and apoptosis in endometrium and endometriosis.Methods: Eutopic and ectopic human endometrium during the menstrual cycle were obtained from 61 patients. Bcl 2 was examined by immunohistochemical techique. Apoptotic cells were detected by TUNEL technique in 34 patients. Results: Apoptotic cells appeared as scatted grandular in secretory phase. The staining of Bcl 2 protein in cytoplasm of glandular cells was mainly seen in proliferative phase. It rarely appeared in secretory phase and absent during menstruation. It appeared highly in endometriosis. Conclusion: Apoptosis of endometrial cell is a cyclic event, which is closely correlated with expression of Bcl 2 during menstruation. The resistance of endometriotic cells to apoptosis may be fundamental in the etiology and pathophysiology of endometriosis.