双酚A在染毒雄性大鼠血清中时间-浓度关系

目的 探讨经口给予雄性大鼠双酚A(bisphenol A,BPA)后,血清中BPA时间-浓度关系.方法 66只无特定病原体(specific pathogen free,SPF)级雄性SD大鼠应用数字表法随机分为10个实验组和1个对照组,每组6只.各实验组大鼠一次性灌胃给予300 mg/kg剂量的BPA后,在0.5、1、2、4、6、12、24、36、60、84 h时间点各采集一组大鼠血液,分离出血清,用荧光-高效液相色谱法检测血清中游离型和总BPA浓度.结果 大鼠一次性经口染毒后,1 h血清中总BPA浓度最高,达17.13μg/ml,2 h血清中BPA浓度急剧下降,24 h血清中总BPA浓度又上升到15.18μg/ml,持续观察84 h,血清中BPA仍有0.51μg/ml.游离型BPA含量较低,染毒0.5 h,血清中游离型BPA浓度为0.57 μg/ml,而后急剧下降,1 h下降到0.06μg/ml,4 h下降到0.03μg/ml,36 h仅有0.01μg/ml.在染毒0.5 h,游离型BPA在血清中所占的百分率最高,为血清总BPA的4.15%(0.57/13.73),其他各时间点血清中游离型BPA所占的百分率均较低.结论一次性经口给予大鼠BPA后,血清中主要以结合型BPA存在,血清总BPA随染毒时间的延长出现双峰现象,可能与BPA代谢过程中肝肠循环有关.

The time-concentration relationship of serum bisphenol A in male rats

Objective To investigate the relationship of time-concentration of bisphenol A (BPA) in male Sprague-Dawley (SD) rats after single oral BPA administration. Methods A total of 66 specific pathogen free (SPF) SD male rats were divided into 10 experimental groups and control group (n =6). The experimental group rats were treated with BPA of 300 mg/kg by oral garage and blood samples were taken from one group at 0. 5,1,2,4,6, 12,24,36,60,84 h time point after oral administration, respectively. The serum BPA concentration was determined by fluorescence-high performance liquid chromatography(FL-HPLC) analysis. Results After oral administration of 300 mg/kg, the total serum BPA concentration of 17. 13 μg/ml was the highest in rats at 1 h,then decreased,but it increased to 15.18 μ/ml again at 24 h,then gradually decreased to 0. 51 μg/ml at 84 h. The level of serum free BPA was lower than that of total serum BPA after oral administration, the serum free BPA was 0. 57μg/ml at 0. 5 h after oral administration. The serum free BPA level decreased to 0. 06 μg/ml at 1 h,0. 03 μg/ml at4 h,0.01 μg/ml at 36 h after oral administration. The free BPA was only 4. 15% (0. 57/13.73) in total BPA in serum at 0. 5 h after oral administration of 300 mg/kg BPA. Conclusion These results suggested that conjugated BPA was the main metabolite of BPA in rat serum after single oral administration. Enterohepatic circulation of BPA glucuronide in rats may results in two peak levels of total BPA in serum.