膀胱癌微卫星不稳定性研究的意义

目的 探讨膀胱移行细胞癌（TCC）中微卫星不稳定性改变（MSI）与膀胱癌临床病理特征的关系,分析其在肿瘤发生、演变过程中的作用,评价其在膀胱癌的诊断及预后中的价值.方法 选择5个微卫星位点,应用PCR-变性聚丙烯酰胺凝胶-银染法,分析36例膀胱癌组织中的微卫星改变.结果 36例中有29例（80.56%）出现微卫星不稳定性改变（LOH/MSI）,其中9号染色体上微卫星位点阳性改变者19例,检出率52.78%;膀胱癌组织微卫星不稳定性改变与肿瘤的分期、分级无关（P＞0.05）.结论 膀胱癌组织中存在微卫星不稳定性改变,5个微卫星位点的诊断组合对膀胱癌的检出率较高,以其分析该地区的膀胱癌具有一定意义,可作为临床上客观评价、筛查和诊断膀胱癌的指标之一.

Microsatellite instability in traditional cell carcinoma of the bladder

Objective To investigate the microsatellite instability (MSI) in traditional cell carcinoma of the bladder, and theirs relationship with the clinic pathological Analysis its function in the development of the turn, evaluate its value in TCC diagnose and prognosis. Methods5 microsatellite markers were selected to examine 36 TCC patients with PCR the loss of heterozygosity (LOH). Results MSI changing was found in 29 of 36 TCC patients (80. 56%), Among them, 19 samples of microstatellite change have been checked out which lie in number 9 chromosome, Checking out rate was 52. 78%. MSI changing of TCC (LOH/MSI) was not significantly related to the tumor grade and tumor stage (P>0. 05). Conclusion MSI has a change in TCC, The synthesizing of 5 microsatellite index sign a-bout TCC have high rate of check out, have some value to analysis the bladder cancer of this arer, it can be used as one of the signs in examination and screening test in clinical for the urine bladder cancer.