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| 白花蛇舌草总黄酮对黑色素瘤A375和B16细胞增殖和凋亡的影响 | |
| 引用本文： | 杨娴,刘汝青,杜德荣,李其林,郑定辉. 白花蛇舌草总黄酮对黑色素瘤A375和B16细胞增殖和凋亡的影响[J]. 岭南皮肤性病科杂志, 2011, 18(6): 359-362 |
| 作者姓名： | 杨娴 刘汝青 杜德荣 李其林 郑定辉 |
| 作者单位： | 1. 广州市红十字会医院皮肤科,广东,广州,510220 2. 中山大学公共卫生学院,广东,广州,510080 3. 广州市东升医院皮肤科,广东,广州,510120 |
| 基金项目： | 广东省中医药局科研课题（2009013）; 广州市中医药中西医结合科研课题（2009-B-16） |
| 摘 要： | 目的：观察白花蛇舌草总黄酮（FOD）对体外培养黑色素瘤A375和B16细胞增殖和凋亡的影响。方法：体外培养黑色素瘤A375和B16细胞,使用不同浓度的FOD作用24 h、48 h,采用MTT比色法检测细胞增殖的抑制作用,流式细胞仪测定细胞凋亡的影响。结果：FOD抑制黑色素瘤A375和B16细胞增殖和诱导细胞凋亡的作用呈明显的浓度和时间依赖性。结论：FOD能有效抑制黑色素瘤A375和B16细胞细胞增殖,并诱导细胞凋亡。 |
| 关 键 词： | 白花蛇舌草总黄酮 黑色素瘤 细胞增殖 细胞凋亡 |
Effects of FOD on the Proliferation and Apoptosis of Melanoma A375 and B16 Cells |
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| YANG Xian,LIU Ru-qing,DU De-rong,LI Qi-lin,ZHENG Ding-hui. Effects of FOD on the Proliferation and Apoptosis of Melanoma A375 and B16 Cells[J]. Southern China Journal of Dermato-Venereology, 2011, 18(6): 359-362 | |
| Authors: | YANG Xian LIU Ru-qing DU De-rong LI Qi-lin ZHENG Ding-hui |
| Affiliation: | (Department of Dermatology,Guangzhou Red Cross Hospital,Guangzhou 510220,China) |
| Abstract: | Objective：To investigate the effects of FOD on the proliferation and apoptosis of A375 and B16 cells in vitro. Methods：The effect of FOD in different concentration on the proliferation of A375 and B16 cells was detected by MTT assay.The effect of FOD on the apoptotic ratio of A375 and B16 cells was detected by flow cytometry. Results：FOD could obviously inhibit the proliferation of A375 and B16 cells in concentration-and time-dependent manner.The apoptotic ratio of A375 and B16 cells was obviously increased by FOD. Conclusion：FOD can inhibit the proliferation and induce apoptosis of A375 and B16 cells. |
| Keywords: | FOD Melanoma Cell proliferation Cell apoptosis |
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