| RP-HPLC法测定参芪肝康胶囊中水飞蓟宾的含量 |
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| 引用本文： | 王婷,于雪,李飞. RP-HPLC法测定参芪肝康胶囊中水飞蓟宾的含量[J]. 实用药物与临床, 2011, 14(6): 492-493 |
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| 作者姓名： | 王婷 于雪 李飞 |
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| 作者单位： | 1. 沈阳药科大学高等职业技术学院,沈阳,110016
2. 中国医科大学附属盛京医院药学部,沈阳,110004 |
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| 摘 要： | 目的建立反相高效液相色谱法测定参芪肝康胶囊中水飞蓟宾的含量。方法色谱柱:Dia-monsil C18(200 mm×4.6 mm,5μm);流动相:甲醇-0.5%醋酸(48∶52,v/v);流速:1.0 mL/min;柱温:25℃;检测波长287 nm。结果水飞蓟宾的线性范围:12.0～240μg/mL(r=0.999 7),平均回收率为97.0%(RSD=1.9%)。结论该方法专属性强,结果准确,可用于参芪肝康胶囊的质量控制。
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| 关 键 词： | RP-HPLC 参芪肝康胶囊 水飞蓟宾 |
Simultaneous determination of silybin in shenqigankang capsules by RP-HPLC |
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| WANG Ting,YU Xue,LI Fei. Simultaneous determination of silybin in shenqigankang capsules by RP-HPLC[J]. Practical Pharmacy and Clinical Remedies, 2011, 14(6): 492-493 |
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| Authors: | WANG Ting YU Xue LI Fei |
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| Affiliation: | 1(1.Vocational and Technical School,Shenyang Pharmaceutical University,Shenyang 110016,China;2.Department of Pharmacy,Shengjing Hospital of China Medical University,Shenyang 110004,China) |
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| Abstract: | Objective To establish a RP-HPLC method for the determination of silybin in Shenqigankang capsules.Methods A Diamonsil C18 column(200 mm×4.6 mm,5 μm)was used.The mobile phase:methanol-0.5% glacial acetic acid solution(48∶ 52,v/v),and the flow rate was 1.0 mL/min.Detection was performed at wavelength of 287 nm under 25 ℃.Results The calibration curves were linear in the ranges of 12.0~240 μg/mL(r=0.999 7).The average recovery rate was 97.0%(RSD=1.9%).Conclusion This method is accurate and the exclusive for quality control of Shenqigankang capsules. |
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| Keywords: | RP-HPLC Shenqigankang capsules Silybin |
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