| CHO-EPO-EGFR细胞株的构建及在光固定EGF材料上的生长 |
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| 引用本文： | 关燕清. CHO-EPO-EGFR细胞株的构建及在光固定EGF材料上的生长[J]. 生物医学工程学杂志, 1999, 0(Z1) |
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| 作者姓名： | 关燕清 |
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| 作者单位： | 华南师范大学生物技术研究所!广州510631 |
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| 摘 要： | 采用人工细胞法 ( liposome)将两种质粒 p CEPO及 p RC/ CMV/ EGFR转染宿主细胞 -中国仓鼠卵巢细胞 ( CHO) ,得到表达水平稳定 ,形态良好的细胞株 CHO- EPO- EGFR,研究该细胞株在光固定EGF的聚苯乙烯材料上生长情况
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| 关 键 词： | 人工细胞法 促红细胞生长因子(EPO) 中国仓鼠卵巢细胞(CHO-K1) 表皮细胞生长因子受体(EGFR) 细胞生长活性 |
The construction of CHO-EPO-EGFR cells and Cells growth on the hoto immobilization of Epidermal Growth Factor polystyrene plate |
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| Guan Yanqing. The construction of CHO-EPO-EGFR cells and Cells growth on the hoto immobilization of Epidermal Growth Factor polystyrene plate[J]. Journal of biomedical engineering, 1999, 0(Z1) |
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| Authors: | Guan Yanqing |
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| Abstract: | The liposome method was used to transfect two plasmid pCEPO and pRC/CMV/EGFR to Chinese hamster ovary cell (CHO K1).A cell line (CHO EPO EGFR) with high level and stable expression was obtained. The cells were cultured under the serum free conditions,and cells growth activity was investigated compared with CHO K1. |
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| Keywords: | liposome EPO Chinese hamster ovary cells (CHO-K1)Epidermal Growth Factor Receptor |
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