Thapsigargin对SLE患者T细胞TCR／CD3复合物介导的[Ca^2＋]i反应的影响

目的证实SLE患者T细胞功能异常是否与TCR/CD3复合物介导的Ca2+]i反应异常有关,以及Ca2+]i反应异常的原因.方法用CD3单抗与羊抗鼠二抗IgG相关联刺激T细胞,并用Thapsigargin干预后,分别用粘附细胞仪连续观察10 min T细胞Ca2+]i的变化,并评价Ca2+]i反应与CD3分子表达的相关性.结果正常人和SLE患者T细胞Ca2+]i反应的基准值相似(P=0.105);SLE患者T细胞的Ca2+]i反应高峰值、平台值明显高于正常对照(P＜0.001,P＜0.001);加入Thapsigargin后二者Ca2+]i反应无显著差异,二者的T细胞CD3阳性率无差异(P=0.665).结论SLE患者T细胞TCR/CD3介导的Ca2+]i反应存在异常,并且这种异常不是因为胞内Ca2+库排空后跨膜Ca2+内流不同所致.

Effect of thapsigargin on TCR/CD3 complex- mediated [Ca2+ ]i responses in T cells of SLE

Objective: To determine whether the primary dysfunction of T cells of SLE was related to abnormal Ca 2+]i responses, and the reason of the abnormal Ca 2+]i response. Methods: After cross-linking of anti-CD3 mAb to sheep anti-mouse IgG and stimulation of T cells, the changes of free calcium ion within T cells and the changes under interference of thapsigargin were observed successively for 10 minutes by an adhesion cytometry. The relation between Ca 2+]i response in T cells of SLE and CD3 expression was evaluated.Results:The baseline Ca 2+]i response in T cells of SLE patients was similar to that in normal control (P=0.105). The peak and plateau Ca 2+]i responses were significantly higher in the group of SLE patients than in normal control (P<0.001,P<0.001). Ca 2+]i responses were similar in both individuals after adding thapsigargin, and the percentage of CD3 + cells was similar in both groups (P=0.665). Conclusion: TCR/CD3-mediated Ca 2+]i response in T cells of SLE was abnormal, which can not be attributed to the difference in Ca 2+ influx across the cell surface membrane after emptying intracellular Ca 2+ stores.