人主动脉夹层组织Myc associated factor X的表达及其生物学功能

目的 探究Myc associated factor X(MAX)在主动脉夹层中的表达情况及相关生物学功能。方法 通过RT-PCR和Western blot检测MAX在15例夹层主动脉中层组织中的表达情况。通过腺病毒载体转染人主动脉平滑肌细胞使其过表达MAX。通过CCK-8、细胞流式等实验技术明确过表达MAX对主动脉平滑肌细胞增殖、凋亡的影响。结果 夹层主动脉组织中MAX的mRNA和蛋白表达水平均明显高于正常主动脉组织。过表达MAX可以明显抑制主动脉平滑肌细胞的增殖,并促进其凋亡(P < 0.05)。结论 MAX通过调控主动脉平滑肌细胞的增殖、凋亡可能诱导其丢失并进而在主动脉夹层的发病中发挥作用。

Expression and biological functions of Myc associated factor X in human aortic dissection tissue

Objective: To elucidate the expression level and biological functions of Myc associated factor X (MAX) involved in aortic dissection. Methods: Fifteen dissected and nine normal human aorta samples were collected. MAX expression level in the media of aorta was evaluated by qRT-PCR and Western blot, respectively. The adenovirus vector was used to transfect human aortic smooth muscle cells (HASMCs) for its overexpression of MAX. The effect of overexpressing MAX on proliferation and apoptosis of HASMCs was analyzed by Cell Counting Kit-8 and flow cytometry, respectively. Results: MAX mRNA and protein expression levels were significantly higher in aortic dissection patients, as compared with healthy individuals. In vitro experiment results indicated that overexpression of MAX significantly inhibited the proliferation of HASMCs and promoted its apoptosis. Conclusions: MAX might induce the loss of HASMCs via regulating its proliferation and apoptosis process and thus play an important role in the pathophysiological processes of aortic dissection