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| 重组蛋白与合成肽抗原对戊型肝炎的诊断价值比较 | |
| 引用本文： | 阮冰,王淑颖,陈勇,洪艳,马亦林. 重组蛋白与合成肽抗原对戊型肝炎的诊断价值比较[J]. 中华检验医学杂志, 2004, 27(2): 79-81 |
| 作者姓名： | 阮冰 王淑颖 陈勇 洪艳 马亦林 |
| 作者单位： | 1. 310003,杭州,浙江大学医学院附属第一医院传染科 2. 杭州市第一人民医院 3. 浙江省医学科学院 |
| 基金项目： | 国家自然科学基金资助项目(39670666)，1999年浙江省医药卫生优秀青年科技人才专项科研基金资助项目，2003年浙江省人事厅留学回国资助项目，2000年浙江省科技计划项目，2000年浙江省医药卫生重点科技项目(2000ZD006) |
| 摘 要： | 目的 比较重组蛋白与合成肽抗原建立的酶联免疫吸附试验 (ELISA)在戊型肝炎实验室诊断中的应用价值。方法 选择戊型肝炎病毒第二开放读码框架 (ORF2 ) ,分别采用重组蛋白及合成肽抗原 ,建立酶联免疫吸附试验 (ELISA) ,同时检测 97份戊型肝炎急性期患者 ,4 7份甲、乙、丙、丁、庚型肝炎患者及 12 0份健康献血者的血清抗 HEV。结果 重组蛋白ELISA、合成肽ELISA与Genelabs重组抗原ELISA试剂盒检测结果的总符合率分别为 96 .9%和 92 % ;重组蛋白ELISA与合成肽ELISA的一致率为 91% ;合成肽ELISA对重组蛋白ELISA的灵敏度为 90 % ,特异度为 10 0 %。结论 戊型肝炎病毒相同表位的重组蛋白和合成肽抗原用于抗 HEVELISA检测有较好的一致性。 |
| 关 键 词： | 肝炎 戊型 肝炎抗体 酶联免疫吸附测定 |
| 修稿时间： | 2003-07-03 |
Comparison of the diagnostic implication between ELISA by recombinant protein and synthetic peptides for detecting antibody to hepatitis E virus |
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| RUAN Bing,WANG Shu-ying,CHEN Yong,Hong Yan,MA Yi-lin. Comparison of the diagnostic implication between ELISA by recombinant protein and synthetic peptides for detecting antibody to hepatitis E virus[J]. Chinese Journal of Laboratory Medicine, 2004, 27(2): 79-81 | |
| Authors: | RUAN Bing WANG Shu-ying CHEN Yong Hong Yan MA Yi-lin |
| Affiliation: | RUAN Bing*,WANG Shu-ying,CHEN Yong,Hong Yan,MA Yi-lin. *Department of Infectious Diseases,First Affiliated Hospital,Medical College of Zhejiang University,Hangzhou 310003,China |
| Abstract: | Objective To evaluate the diagnostic implication of ELISA by recombinant protein or synthetic peptides for detecting antibody to hepatitis E virus (HEV). Methods Based on recombinant protein or synthetic peptides from the open reading frame (ORF) 2 of HEV, enzyme-linked immunosorbent assay (ELISA) was developed separately for detecting anti-HEV among 97 sera of acute-phase patients with hepatitis E.and 47 sera of patients with hepatitis A,B,C,D or G Results As comparing with Genelabs ELISA kit,the coincident rate for recombinant protein ELISA was 96.9% and for synthetic paptides ELISA 92%.The consistent rate between recombinant protein ELISA and synthetic peptides ELISA was 91%. Compared to recombinant protein ELISA and synthetic peptides ELISA showed 90% of sensitivity and 100%of specificity.Conclusion Used as a diagnostic antigen in anti-HEV ELISA test, the recombinant protein is identical with the synthetic peptides which come from the same epitope region. |
| Keywords: | Hepatitis E Hepatitis antibody Enzyme-linked immunosorbent assay |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |