KGF对无排卵型功血子宫内膜上皮细胞ER和PR的调节研究

目的:观察角质细胞生长因子(KGF)对离体培养的无排卵型功血子宫内膜上皮细胞(EEC)中雌激素受体(ER)和孕激素受体(PR)表达的影响.方法:体外培养无排卵型功血子宫内膜上皮细胞,加入不同浓度的角质细胞生长因子作用后,RT-PCR检测ER和PR的mRNA表达改变,并与在体无排卵型功血子宫内膜组织及培养的正常子宫内膜上皮细胞进行比较.结果:RT-PCR方法检测两组ER和PR的mRNA的表达情况,结果显示功血组较对照组均显著增高(P<0.05).结论:KGF对体外培养的EEC有增加ER和PR的作用,但程度不如正常的子宫内膜上皮细胞,提示无排卵功血可能存在与正常的子宫内膜上皮细胞不同的生长机制,但二者的作用均受KGF的影响.

Effect of keratinocyte growth factor on expression change of ER and PR in cultured endometrial epithelial cells

Objective：To observe the effect of keratinocyte growth factor（KGF）on estrogen receptor（ER）and progesterone receptor （PR）expression of cultured endometrial epithelial cells（EECs）of anovulatory dysfunctional uterine bleeding（ADUB）in vitro.Methods：The EECs of ADUB were separated and cultured with different concentrations of KGF.ER and PR mRNAs were determined by RT-PCR technique.These results were compared with normal EECs and EECs of endometrium.Results：KGF（50ng/mL）increased mRNA expressions of both ADUB and normal EECs by RT-PCR method（P〈0.05）.But the degrees of increase in ADUB group were lower than that of normal group.Conclusion：KGF can increase ER and PR expressions of cultured ADUB EECs.But the enhancement in ADUB was lower than that of normal EECs.These suggest that ADUB EECs have different growing mechanism with normal EECs,and both are affected by KGF.