儿童血镉水平对血清中SOD NO NSE的影响

目的:观察不同血镉水平对儿童血清超氧化物歧化酶(SOD)、一氧化氮(NO)和神经元特异性烯醇化酶(NSE)的影响.方法:在儿童保健门诊选择健康儿童50例,男26例,女24例,用无火焰石墨炉原子吸收光谱法测定血镉浓度,按血镉含量分为正常组、低镉组和高镉组.同时测定血清中SOD活力、NO含量和NSE浓度.结果:血镉含量正常组(血镉<2.0 μg/L)17例,占34%,低镉组(血镉2.0～4.0 μg/L)21例,占42%,高镉组(血镉>4 μg/L)12例,占24%,随着血镉浓度的升高,SOD活力、NO含量呈下降趋势,并有显著负相关(分别为r=-0.32,t=2.37,P<0.05;r=-0.46,t=3.58,P<0.01).随着血镉浓度的升高,NSE浓度呈上升趋势,两者有显著正相关关系(r=0.48,t=3.75,P<0.01).结论:血清中SOD活力和NO含量下降及NSE浓度的升高可作为临床上判断儿童早期镉损伤的重要指标.

Effect of blood cadmium level on serum SOD,NO and NSE of childhood

Objective：To observe the effects of blood cadmium level on serum SOD,NO and NSE of childhood,and to probe into its mechanism.Methods：50 healthy children（26 boys and 24 girls） were divided into 3 groups：normal group,low cadmium group and high cadmium group,according to their blood cadmium level.Blood cadmium levels were measured with flameless AAS.The activity of SOD,the contents of NO and NSE in serum were also detected.Results：The normal（2.0 μg/L）,lower（2.0-4.0 μg/L） and higher（4.0 μg/L） blood cadmium levels were respectively 17（34%）,21（42%） and 12（24%） cases respectively.The activity of SOD and the content of NO decreased with the increase of blood cadmium level,showing the negative correlation with the blood cadmium level（respectively r=-0.32,t=2.37,P0.05;r=-0.46,t=3.58,P0.01,respectively）;as the blood cadmium levels increasing,the contents of NSE showed increasing trend,demonstrating the significant positive correlation（r=0.48,t=3.75,P0.01）.Conclusion：The lowering of the activity of SOD and content of NO,and elevation of NSE in serum might be taken as the important indicators of clinical criteria in determining early adverse effects of cadmium in children.