大鼠颊黏膜鳞癌细胞系端粒酶活性与其转移相关性的研究

目的：检测SD大鼠颊黏膜鳞癌细胞系SDSCC—B与其单克隆细胞系Rca—B细胞的端粒酶活性，分析其对细胞转移能力的影响。方法：采用单细胞克隆培养法筛选细胞系SDSCC—B，建立单克隆来源的细胞系Rca—B；分别检测2个细胞系端粒酶的活性：以Tca8113和Tb细胞系做阳性对照。2个细胞系经裸鼠尾静脉注射后，观察其实验性肺转移情况。检测结果采用SPSS11．0．1软件包分别进行q检验和t检验。结果：端粒酶活性检测结果显示，2个细胞系的端粒酶活性均为强阳性．显著高于大鼠正常颊黏膜细胞，差别均具有统计学意义（P〈0．01）；Rca—B端粒酶活性高于SDSCC—B细胞，差别具有统计学意义（P〈0．05）；同时。Rca—B细胞实验性肺转移能力较后者高，Rca—B组与SDSCC—B组肺重，体重的比值差别具有统计学意义（P〈0．05）。结论：SD大鼠细胞系SDSCC—B和Rca—B均具有高转移特性，端粒酶活性的表达与转移能力有关，Rca—B的转移能力更强。

The relationship of telomerase activation and metastasis of buccal squamous cell carcinoma cells in rats

PURPOSE:To study the relationship of the telomerase activity and the potential of experimental metastasis to the lung between Sprague-Dawley rat buccal squamous cell carcinoma cell line and its purified sub-cell line. METHODS:In vitro inbred line Rca-B was purified from SDSCC-B cell line by using mono-cell cloning method. The telomerase activity of cell lines was analyzed by TRAP-ELISA. The ratios of LW/BW (lung weight/body weight)of nude mice after experimental lung metastasis were used to evaluate the metastatic characters. The results were analyzed by SPSS11.0.1 software package for q test and t test respectively. RESULTS: The results showed that the telomerase activity of the cell lines was significantly higher than normal buccal mucosa cells(P<0.01), telomerase activity of Rca-B cells was significantly higher than that of SDSCC-B cells(P<0.05). The ratios of LW/BW of nude mice of the two cell lines were significantly different(P<0.05). CONCLUSION:Rca-B and SDSCC-B cells were of high metastatic ability, and Rca-B was more easily metastatic and lower differenciated .The expression of telomerase activity was relevant with the metastatic ability of these cells. Supported by Key Project of National Natural Science Foundation of China (Grant No.30330580) and Shanghai Key Scientific and Technological Project (Grant No.014319359).