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| 鼻咽癌组织中EB病毒的不同原位检测方法比较 | |
| 引用本文： | 许亮国,姚开泰,谢鹭,周文,杨玉文,陈曲侯,王()章. 鼻咽癌组织中EB病毒的不同原位检测方法比较[J]. 癌症, 2000, 19(1): 31-33 |
| 作者姓名： | 许亮国 姚开泰 谢鹭 周文 杨玉文 陈曲侯 王()章 |
| 作者单位： | 1. 湖南医科大学卫生部癌变原理重点实验室,湖南长沙410078;中山大学生命科学院,广东广州510275 2. 湖南医科大学卫生部癌变原理重点实验室,湖南长沙410078 3. 中山大学生命科学院,广东广州510275 |
| 基金项目： | 中国科学院资助项目，39730200， |
| 摘 要： | 目的：探讨在鼻咽癌组织石蜡切片标本中原位检测ＥＢ病毒的更快速、更灵敏的方法。方法：用地高辛标记的寡核苷酸ＥＢＥＲ－１和ＥＢＶ ＤＮＡ的ＢａｍＨＩ－Ｗ片段为探针，分别用原位杂交、原位ＰＣＲ检测鼻咽癌组织切片中ＥＢＶ的分布情况。结果：用寡核苷酸ＥＢＥＲ－１作探针原位杂交检测ＥＢ病毒的方法比用ＢａｍＨＩ－Ｗ片段作探鱼原位杂交及原位ＰＣＲ的方法更灵敏、更简单。结论：用ＥＢＥＲ－１作探鱼原位杂交检测鼻咽癌组 |
| 关 键 词： | 原位杂交 EBER－1 EB病毒 鼻咽肿瘤 |
| 文章编号： | 1000－467X(2000)01－0031－03 |
| 修稿时间： | 1999－03－10 |
Comparison of different methods for in situ detection of Epstein-Barr virus in nasopharyngeal carcinoma |
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| XU Liang-guo,YAO Kai-tai,XIE Lu,et al.. Comparison of different methods for in situ detection of Epstein-Barr virus in nasopharyngeal carcinoma[J]. Chinese journal of cancer, 2000, 19(1): 31-33 | |
| Authors: | XU Liang-guo YAO Kai-tai XIE Lu et al. |
| Abstract: | Objective: To investigate a rapid, sensitive non-isotopic method for in situ detection of Epstein-Barr virus (EBV) in paraffin-embedded section of nasopharyngeal carcinoma (NPC) tissues. Methods: The oligo-nucleotide EBER-1 and EBV BamHI-W fragment were labeled with digoxigenin as probes, in situ hybridization and PCR in situ hybridization were performed to detect EBV distribution in NPC tissues. Results: The signal strength of in situ hybridization with EBER-1 oligonucleotide probe was clearly stronger than that of in situ hybridization and PCR in situ hybridization with BamHI-W fragment probe. Conclusion: In situ hybridization with EBER-1 probe to detect EBV in NPC tissues is a sensitive, simple and rapid method. |
| Keywords: | In situ hybridization In situ PCR EBER-1 Epstein-Baar virus |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |