| 小鼠骨髓间充质干细胞分离培养方法的建立 |
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| 引用本文： | 于美娟,张雅妮,冯善伟,熊符,张成.小鼠骨髓间充质干细胞分离培养方法的建立[J].解剖学研究,2009,31(6):424-427. |
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| 作者姓名： | 于美娟 张雅妮 冯善伟 熊符 张成 |
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| 作者单位： | 1. 广州医学院第二附属医院,广州医学院神经科学研究所,广东,广州,510260
2. 中山大学附属第一医院神经科 |
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| 基金项目： | 广州医学院博士启动基金 |
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| 摘 要： | 目的建立培养小鼠骨髓间充质干细胞(none mesenchymal stem cells,BMSCs)的分离培养方法。方法采用全骨髓体外分离并采用差速贴壁法纯化扩增C57BL/6小鼠BMSCs,形态学观察细胞生长情况,流式细胞仪检测其表面抗原情况并观察其多项分化潜能。结果使用该方法纯化扩增的BMSCs形态均一,生长良好,表达CD29、CD44和Sca-1,不表达CD11b、CD45和CD34,并具有成骨成脂潜能。结论通过全骨髓贴壁法可以成功的分离培养出小鼠BMSCs。
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| 关 键 词： | 骨髓间充质干细胞 小鼠 细胞培养 鉴定 |
Isolation and cultivation of mice bone marrow mesenchymal stem cells |
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| YU Mei-juan,ZHANG Ya-ni,FENG Shan-wei,XIONG Fu,ZHANG Cheng.Isolation and cultivation of mice bone marrow mesenchymal stem cells[J].Anatomy Research,2009,31(6):424-427. |
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| Authors: | YU Mei-juan ZHANG Ya-ni FENG Shan-wei XIONG Fu ZHANG Cheng |
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| Institution: | YU Mei-juan,ZHANG Ya-ni,FENG Shan-wei,XIONG Fu,ZHANG Cheng.Institute of Neurosciences,The Second Affiliated Hospital of Guangzhou Medical College Guangzhou510260 China |
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| Abstract: | Objective To establish one method of isolation and cultivation of C57 BL/6 mice BMSCs in vitro.Mehthods BMSCs were isolated, cultured through the whole bone marrow method, amplified by the differential adherent method. Morphology was observed.The expression of superficial antigen was examined by flow cytometry and whether they had multi differentiation potentions was tested.Results By this method, BMSCs growed well.The cells showed positive with CD29, CD44 and Sca-1, but showed negative with CD1 l b, CD45 a... |
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| Keywords: | Bone mesenchymal stem cells Mice Cell culture Identify |
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