甲真菌病患者多部位红色毛癣菌分离株的DNA分型

目的探讨甲真菌病患者多部位红色毛癣菌感染不同部位分离菌株基因型的差异。方法采用PCR扩增红色毛癣菌rDNA非转录间隔区(NTS)中Trs-1片段,检测基因多态性并比较。结果30株受试菌株按照PCR指纹图共分为3型,基因型分布与感染部位无相关。10例受试患者中,5例不同感染部位分离出的菌株基因型存在差异。结论甲真菌病患者多部位红色毛癣菌感染可能为多菌株引起,部分患者可能存在多菌株的混合感染。

Molecular Typing of Trichophyton Rubrum Isolated from Different Lesions in the Same Patients with Onychomycosis

Objective To determine whether Trichophyton rubrum isolated from different lesions in the same patient with onychomycosis was of different strains.Methods DNA was extracted from the isolates,then subjected to a polymerase chain reaction-based typing which analyzed the number of repetitive elements in the non-transcribed spacer region of the ribosomal DNA gene repeats.Results Thirty strains of T.rubrum were isolated from 10 patients with fungal infection on multiple sites.All strains could be classified into 3 genotypes.The genotypes distribution was unrelated to sites of infection.It happened to 5 of the 10 patients that multiple genotypes were involved in T.rubrum on different sites in the same body.Conclusion The study shows that multiple genotypes are involved in T.rubrum infection on different sites in the same patient with onychomycosis,suggesting external sources of infection rather than infection from a different site in the same individual.