| 人无排卵功血子宫内膜上皮细胞生物学特性初步研究 |
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| 引用本文： | 王欣,程湘,成娅,陈波洁,刘萍,王智,林桂兰,杨合荣,王惠. 人无排卵功血子宫内膜上皮细胞生物学特性初步研究[J]. 第三军医大学学报, 2006, 28(20): 2050-2053 |
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| 作者姓名： | 王欣 程湘 成娅 陈波洁 刘萍 王智 林桂兰 杨合荣 王惠 |
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| 作者单位： | 第三军医大学,新桥医院妇产科,重庆,400037;第三军医大学,西南医院妇产科,重庆,400038 |
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| 摘 要： | 目的 探讨ADUB EEC的部分生物学特性.方法 采用电镜、MTT、磁酶免及免疫组化等方法了解ADUB EEC的超微结构、生长曲线、CA125分泌及PCNA、ER、PR表达情况,并与在体ADUB子宫内膜组织及培养的正常EEC进行比较.结果 体外培养的ADUB EEC基本保留了体内的结构特征,体外增生水平低,分泌异常.结论 原代培养的ADUB EEC为研究无排卵功血发病机制及治疗奠定了实验基础.
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| 关 键 词： | 无排卵功血 子宫内膜 上皮细胞 原代培养 |
| 文章编号： | 1000-5404（2006）20-2050-04 |
| 收稿时间： | 2005-11-14 |
| 修稿时间： | 2005-11-142006-01-05 |
Primary study on biological properties of human endometrial epithelial cells from anovulatory dysfunctional uterine bleeding patients |
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| WANG Xin,CHENG Xiang,CHNEG Ya,CHEN Bo-jie,LIU Ping,WANG Zhi,LIN Gui-lan,YANG He-rong,WANG Hui. Primary study on biological properties of human endometrial epithelial cells from anovulatory dysfunctional uterine bleeding patients[J]. Acta Academiae Medicinae Militaris Tertiae, 2006, 28(20): 2050-2053 |
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| Authors: | WANG Xin CHENG Xiang CHNEG Ya CHEN Bo-jie LIU Ping WANG Zhi LIN Gui-lan YANG He-rong WANG Hui |
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| Affiliation: | 1. Department of Obstetrics and Gynecology, Xinqiao Hospital, Third Military Medical University, Chongqing 400037 ; 2.Department of Obstetrics and Gynecology, Southwest Hospital, Third Military Medical University, Chongqing 400038, China |
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| Abstract: | Objective To investigate the biological properties of endometrial epithelial cells(EEC) from anovulatory dysfunctional uterine bleeding(ADUB)patients. Methods EEC were isolated from 30 diagnosed ADUB patients, and from 22 patients with normal menstruation. The growth curve, ultrastructure, CA 125 production, and the expressions of proliferative cell nuclear antigen (PCNA), estrogen receptor (ER) and progesterone receptor (PR) of those cells were respectively investigated with Metromethyl Thiazolyl Blue assay (MTT), electron microscopy, MAIA and immunocytochemistry. Results ADUB EEC in vitro retained pathologic configuration of in vivo cells such as retarded growth and abnormal secretion. Conclusion The isolation and primary culture of ADUB EEC can serve to be one of the in vitro models of ADUB. |
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| Keywords: | abnormal dysfunctional uterine bleeding endometrium epithelial cell primary cell culture |
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