VEGF-C及VEGFR-3在结直肠癌组织中的表达及意义

目的探讨血管内皮生长因子-C(VEGF-C)、血管内皮生长因子受体-3(VEGFR-3)在结直肠癌组织中的表达及其与临床病理参数之间的关系。方法采用免疫组织化学法,检测63例结直肠癌患者(病例组)及20例良性腺瘤及周围正常结肠组织(对照组)中微淋巴管密度(LMVD)及VEGF-C、VEGFR-3的表达;分析LMVD、VEGF-C和VEGFR-3表达与临床各病理参数之间的关系。结果病例组LMVD高于对照组(14.82±4.41 vs 9.56±2.32.P<0.01),病例组中LMVD在有淋巴结转移、TNM分期较高、VEGF-C和VEGFR-3阳性表达的结直肠癌组织中表达较高,差异有统计学意义(P<0.01),而不同性别、年龄、组织分型和分化程度的患者中差异无统计学意义(P>0.05)。VEGF-C和VEGFR-3在病例组的表达均高于对照组(分别为60.32%vs 35.00%和71.43%vs 40.00%,均为P<0·01),病例组VEGF-C和VEGFR-3的表达在有淋巴结转移和TNM分期较高的患者中明显升高,差异有统计学意义(P<0.01),而在不同性别、年龄、组织分型和分化程度的患者中差异无统计学意义(P>0.05)。结论 VEGFC和VEGFR-3与结直肠癌的发展及转移密切相关,其阳性表达率高于结直肠良性肿瘤及其周围正常组织,有可能为结直肠癌的治疗提供新的靶点。

Expression and significance of VEGF-C and VEGFR-3 in colorectal cancer tissue

Objective To investigate the expression of VEGF-C and VEGFR-3 in colorectal cancer and to analyze the association between VEGF-C or VEGFR-3 expression and clinicopathological variables.Methods Using immunohistochemistry, VEGF-C and VEGFR-3 protein expression was detected in 63 cases of colorectal cancer tissues（experimental group） and 20 cases of benign adenoma and normal tissues（control group）.And lymphatic micro-vessel density（LMVD） was counted respectively.The relationship between VEGF-C,VEGFR-3,LMVD and the clinicopathological variables was analyzed.Results LMVD in experimental group was higher than that in the control group（14.82±4.41 vs.9.56±2.32,P 0.01）. LMVD was significantly higher in patients with lymph node metastasis,,in the later TNM stage,with positive VEGF-C and VEGFR-3 expression（P 0.01）.No significant difference was found between sex,age,histological types and differentiation （P 0.05）.The expressions of VEGF-C and VEGFR-3 in colorectal cancer were significantly higher than those in benign adenoma and normal tissues（60.32%vs.35.00%and 71.43%vs.40.00%,both P 0.01）.In the experimental group,significantly higher level of VEGF-C and VEGFR-3 was observed in the metastatic lymph nodes and in the later TNM stage tissues（P 0.01）.Nevertheless,no significant difference was found between different gender,age,histological types,and differentiation.Conclusion The expressions of VEGF-C and VEGFR-3 in colorectal cancer were higher than those in benign adenoma and normal tissues and closely correlated with tumor progression and invasiveness.This can provide new targets for colorectal cancer therapy.