保留残迹对前交叉韧带移植物腱骨愈合影响的实验研究

目的探讨保留残迹重建前交叉韧带对移植物腱骨愈合的影响。方法 32只新西兰兔一期行双侧前交叉韧带重建术,一侧保留残端纤维,对侧切除残端纤维。重建术后6、12、18及24周时,采用HE染色、甲苯胺蓝染色观测,分析移植物腱骨愈合变化情况。结果重建术后各观察时间点上,保留残迹组移植物腱骨界面组织构建更接近正常,术后24周时保留残迹组腱骨界面软骨细胞含量明显高于切除残迹组[（56.5±2.4）vs（45.7±2.7）,P〈0.05]。结论保留残迹重建前交叉韧带有助于移植物腱骨愈合。

Effect of the attachment remnant preservation on tendon-bone healing in a rabbit model of anterior cruciate ligament reconstruction

Objective To investigate the effect of attachment remnant preservation on the healing of tendon-bone interface in a rabbit model of anterior cruciate ligament （ACL） reconstruction. Methods 32 New Zealand rabbits were involved and both knees of each rabbit received transection of anterior cruciate ligament simultaneously. ACL of one knee was reconstructed with preservation of the residual portion of ligament, and the contralateral one was reconstructed with excision of the remnant. At 6, 12, 18, and 24 weeks after reconstruction, the healing status and morphology of tendon-bone interface were observed in vitro by hemaetoxylin-eosin staining and Toluidine Blue staining. Results At each observation time point, the morphology and structure of tendon-bone interface in the remnant-preserved group were improved similar to normal, and the number of chondrocytes at the interface was significantly increased in the remnantpreserved group compared with that of control group [ （ 56. 5 ± 2.4 ） vs （ 45.7± 2. 7 ）, P 〈 0.05 ]. Conclusions The preservation of attachment remnant in anterior cruciate ligament reconstruction could enhance the healing of tendon-bone interface.