淫羊藿苷对内毒素抑制牙周膜细胞ALP表达的影响

目的：探讨淫羊藿苷对人牙周膜细胞（periodontal ligament cells，PDLC）增殖及对内毒素（Lipopolysaccha-ride，LPS）干扰下碱性磷酸酶（alkaline phosphatase，ALP）的影响。方法：原代培养PDLC，MTT法检测不同浓度（0、10^-5～10^-9mol／L）淫羊藿苷对PDLC增殖的影响；RT—PCR、对硝基苯酚法测定淫羊藿苷对LPS抑制PDLC的ALPmRNA表达和分泌的影响。结果：淫羊藿苷在一定浓度下（10^-6～10^-7mol／L）可促进PDLC增殖；10υg／mLLPS可抑制PDLC的ALP活性；加入10^-6mol／L淫羊藿苷干扰后，对LPS抑制PDLC的ALP有拮抗作用，可提高其mRNA表达和活性。结论：淫羊藿苷在一定浓度时可促进PDLC增殖；可能通过拮抗LPS抑制其ALP的活性。

Effects icariin of on the alkline phosphatase activity of human periodontal ligament cells inhibited by LPS

Objective： To investigate the effects of icariin （ICA） on proliferation of huaman periodontal ligament cells （human periodontal ligament cells, hPDLC） and the alkaline phosphatase （alkaline phosphatase,ALP） activity inhibited by LPS. Method： HPDLC were cultured in virto and stimulated with icariin with different concentrations （0,10^-5,10^-6,10^-7,10^-8, 10^-9 mol / L）） for 96 hours.The ability of proliferation of HPDLC was detected by MTT method.The activity of alkaline phos- phatase and the expression of alkaline phosphatase mRNA was seperately determined by p-Nitropbnyl phosphate （pNPP） method and RT-PCR after being treated with icariin at the concentration of 10^-6 mol / L inhibited by LPS. Result： icariin had a dose-dependent effect on the proliferation the hPDLC in a suitable concentration range from 10^-6～10^-8 mol / L. The alka- line phosphatase activity was remarkably inhibited by incubation of PDLC with 10 υg / mL LPS and was improved in the presence of icariin at the concentration of 10^-6 mol / L. Conclusion： Ieariin have the effect on the proliferation of PDLC and improve the ALP activity inhibited by LPS, which could held for Peri-at）ical tissue regeneration.