Effect of Nuclear Factor-κB on Airway Remodeling in Asthmatic Rats |
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摘 要: |
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关 键 词: | 核因子-κB 气道重塑 动物实验 作用机制 气道高反应 基底膜细胞 哮喘 |
收稿时间: | 2 September 2003 |
Effect of nuclear factor-κB on airway remodeling in asthmatic rats |
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Authors: | Xu Shuyun Xu Yongjian Zhang Zhenxiang Ni Wang Chen Shixin |
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Institution: | (1) Department of Respiratory Medicine, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, 430030 Wuhan, China |
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Abstract: | Summary In order to investigate the effect of nuclear factor-κB (NF-κB) on airway remodeling in asthmatic rats. 18 Wistar rats were
divided into three groups: asthmatic group: pyrrolidine dithiocarbamate (PDTC) group, in which rats were injected intraperitoneally
with NF-κB specific inhibitor PDTC (100 mg/kg) before ovalbumin (OVA) challenge; control group. The NF-κB activity and the
expression of inhibitory protein κBα (I-κBα) in airway were detected by electrophoretic mobility shift assay (EMSA). Western
blot and immunohistochemistry respectively. The infiltration of inflammatory cells, the number of Goblet cells, the area of
collagen and smooth muscle in airway were measured by means of image analysis system. The results showed that with the up-regulation
of airway NF-κB activity in asthmatic group, the number of goblet cells (3.08±0.86/100 μm basement membrane (BM)), the area
of collagen (24.71±4.24 μm2/μm BM) and smooth muscle (13.81±2.11 μm2/μmBM) in airway were significantly increased (P<0.05) as compared with control group (0.14±0.05/100μmBM. 14.31±3.16 μm2/μm BM and 7.67±2.35 μm2/μm BM respectively) and PDTC group (0.33±0.14/100 μmBM, 18.16±2.85 μm2/μm BM and 8.95±2.16 μm2/μm BM respectively). However, there was no significant difference between PDTC group and control group (P>0.05). It was concluded that the activity of NF-κB is increased in airway of asthmatic rats. Inhibition of NF-κB, activation
can attenuate constructional changes in asthma airway, suggesting NF-κB may contribute to asthmatic airway remodeling.
XU Shuyun, female, born in 1970, M. D. Ph. D.
This project was supported by a grant from Foundation for University Key Teacher by the Ministry of Education (2000 year). |
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Keywords: | asthma airway remodeling nuclear factor-kappaB |
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