黄胸鼠对大隆的实验室敏感性测定

目的实验室测定黄胸鼠对第二代抗凝血灭鼠剂大隆的敏感性,为进行抗药性调查提供正常的黄胸鼠敏感种群本底资料。方法按全国鼠类抗药性监测协作组统一方法,做无选择性摄食试验。试鼠雌雄分开,随机分组,分5个不同食毒期摄食0.00016%大隆毒饵。数据用Bliss计算机程序,计算不同食毒期与对应死亡率之间的关系。结果黄胸鼠性别之间对大隆的敏感性差异无统计学意义(Pb>0.05,PLFP50>0.05),故雌雄数据合并计算,得毒力回归方程y=2.49 5.70x,LFP50、LFP99及其95%置信限分别是2.75(2.39～3.17)d和7.04(4.89～10.13)d。结论按WHO修订以致死99%敏感靶标鼠种的食毒期(LFP99)取整天数作为抗药性检验标准,湛江地区黄胸鼠对0.00016%的大隆毒饵食毒期超过8d存活为产生抗药性。

The susceptibility of Rattus tanezumi to brodifacoum in laboratory

Objective To determine the susceptibility of Rattus tanezumi to Brodifacoum in the laboratory.Methods No-choice feed was performed according to the method of rodent resistance monitoring group of China.Firstly,the males and females were separated,then divided into groups at random.The rodent was feed with 0.000 16% brodifacoum bait at 5 different feeding days.The data was analyzed with Bliss software.Results There was no significant difference between the susceptibility of different sex R.tanezumi to Brodifacoum(Pb>0.05,PLFP50>0.05).The virulence regression equation was y=2.49 5.70x,and the LFP50,LFP99 and its 95% confidence limits were 2.75(2.39-3.17)d and 7.04(4.89-10.13)d days respectively.Conclusion According to the revised standard of WHO that the integer of LFP99 value was the resistance distinguish standard,the survivor that were fed with 0.000 16% bait for 8 d was the resistance species in Zhanjiang.