基因芯片研究糖尿病肾病大鼠肾皮质基因表达

目的：研究正常与糖尿病肾病大鼠肾脏的基因表达谱，大规模分析糖尿病时基因表达水平的变化。方法：（1）实验大鼠分为两组，一组为正常对照组，另一组为糖尿病组；（2）从肾皮质中抽提mRNA，经反转录分别用Cy3,Cy5荧光标记，获得两组动物来源的cDNA探针；（3）cDNA探针与Biodoor基因表达谱芯片杂交，结果由扫描仪扫描并用软件进行分析统计。结果（1）18．4％的基因表达谱发生明显的变化，其中12条基因在糖尿病时表达量明显下降，而323条基因在糖尿 时表达量明显上升；（2）已报道基因占了差异表达基因总数的18．8％左右，大多数已报道基因在糖尿病时的表达模式与其他作者的报道类似。结论：利用基因芯片技术结合实验动物模型能大规模，高通是地研究糖尿病的基因表达谱，初步筛选出选出疾病相关基因，对进一步阐明疾病在基因水平上的发病机制有十分重要的作用。

Study on Gene Expression Profile in Renal Cortex in STZ Induced Diabetic Nephropathy Rats by cDNA Microarray (Genechip)

Objective To study the gene expression profile in STZ induced diabetic rats and to analyze differential gene expression patterns between normal and diabetic rats in large scale.Methods (1) Experimental diabetic nephropathy was induced by streptozotocin in S D male rats. Kidney biopsies were obtained from control (citrate injected) and diabetic rats at day 60; (2) The fluorescent labeled DNA probes were prepared from mRNA, which were extracted from both the renal cortex of the control and the diabetic rats; (3) Two kinds of probes were then hybridized against BioDoor Genechip including 1 500 reported genes and 2 500 unreported genes, the fluorescent signals were scanned and analyzed.Results (1) There were about 8.4% genes altered in gene expression profile, the expression level of 12 genes were down regulated while 323 genes were up regulated significantly in renal corted in diabetic rats; (2) The reported genes changed in both the normal and the diabetic rats accounting 18.8% in the total differential expression genes.Conclusion Combined with animal model, gene chip could be used to analyze gene expression profile in diabetes, as well to screen diseases associated genes. So the genechip technology can play an important role in exploring disease mechanism at genetic level.