Calponin-1和Transgelin蛋白在临产前后 妊娠子宫平滑肌中的表达

目的：探讨调宁蛋白1（Calponin-1）和转凝蛋白（Transgelin）在临产前后子宫平滑肌组织中的表达及其在分娩发动中的作用机制。方法：选取14例初产妇的子宫体部及子宫下段平滑肌组织,分为未临产组及临产组。分别用免疫组织化学及Western印迹检测Calponin-1和Transgelin的表达水平。结果：临产组子宫体部和下段平滑肌组织Calponin-1的表达较未临产组明显升高（P<0.05）。临产组子宫体部平滑肌组织中Transgelin的表达较未临产组升高（P<0.05）,而子宫下段平滑肌组织中Transgelin的表达2组差异无统计学意义（P>0.05）。结论：子宫体部和下段平滑肌组织中的Calponin-1和子宫体部平滑肌组织中的Transgelin可能参与了子宫平滑肌收缩的调节,与分娩发动关系密切。

Expression of Calponin-1 and Transgelin in human uterine smooth muscles in non-labor and labor situation

ObjectiveTo investigate the expression of Calponin-1 and Transgenlin in the uterine smooth muscles during normal labor on-sets, and to evaluate their effect on initiating the normal labor.MethodsA total of 14 uterine bodies and lower segments of human pregnancy were divided to a non-labor group (NIL) and a labor group（IL）. Immunohistochemical technology and Western blot were used to determine the expression of Calponin-1 and Transgelin in the 2 groups. ResultsImmunohistochemical detection and Western blot showed that Calponin-1 protein in the uterine smooth muscle tissue of the body and the lower uterine segment of smooth muscle tissues had significant difference (P<0.05). The expression of Transgelin in the uterine body smooth muscle tissue in the IL was higher than that in the NIL(P<0.05). In the lower uterine segments of the smooth muscle, the expression of Transgelin was not significantly different in the 2 groups (P>0.05).ConclusionCalponin-1 of the uterine smooth muscle and Transgelin of the uterine body smooth muscle may involve in the regulation of uterine smooth muscle contractility, which is closely related to child birth launch.