A new type of Schwann cell graft transplantation to promote optic nerve regeneration in adult rats |
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| Authors: | Fang Yuan Mo Xiaofen Guo Wenyi Zhang Meng Zhang Peihua Wang Yan Rong Xianfang Tian Jie Sun Xinghuai |
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| Institution: | Department of Ophthalmology and Vision Science, Eye and ENT Hospital, Shanghai Medical College, Fudan University, Shanghai, People's Republic of China. |
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| Abstract: | Like other parts of the central nervous system, the adult mammalian optic nerve is difficult to regenerate after injury. Transplantation of the peripheral nerve or a Schwann cell (SC) graft can promote injured axonal regrowth. We tried to develop a new type of tissue-engineered SC graft that consisted of SCs seeded onto a poly(lactic-co-glycolic acid)/chitosan conduit. Meanwhile, SCs were transfected along the ciliary neurotrophic factor (CNTF) gene in vitro by electroporation to increase their neurotrophic effect. Four weeks after transplantation, GAP-43 labelled regenerating axons were found in the SC grafts, and axons in the CNTF-SC graft were longer than those in the SC graft. Tissue-engineered SC grafts can provide a feasible environment for optic nerve regeneration and may become an alternative for bridging damaged nerves and repairing nerve defects in the future. |
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| Keywords: | transplants optic nerve injury nerve regeneration Schwann cells gene therapy ciliary neurotrophic factor |
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