Peptide mimotopes of Neisseria meningitidis group B capsular polysaccharide |
| |
Authors: | Park Inho Choi In-Hong Kim Se Jong Shin Jeon-Soo |
| |
Institution: | Department of Microbiology, Brain Korea 21 Project for Medical Science, Yonsei University College of Medicine, 134 Shin chon-dong, Seodaemoon-gu, Seoul 120-752, Korea. |
| |
Abstract: | The antigenic similarity between Neisseria meningitidis group B (NMGB) capsular polysaccharide (PS) and human polysialic acid (PSA) has hampered the development of a NMGB PS-based vaccine. But the possibility of a safe vaccine based on NMGB PS has been demonstrated by the existence of the NMGB PS-associated nonautoreactive epitope, which is distinct from those present on human PSA. To obtain peptide mimotopes of NMGB PS, we used HmenB3, a protective and nonautoreactive monoclonal antibody, to screen a phage library with 12 amino acids. We obtained 23 phage clones that bound to HmenB3 but not in the presence of E. coli K1 PS which is alpha(2-8)-linked PSA like NMGB PS]. The clones contained 3 mimotopes and differed from previously described NMGB PS mimotopes. Immunization with a synthetic peptide of one mimotope elicited anti-NMGB antibodies in BALB/c mice. These mimotopes may be useful in the development of group B meningococcal vaccines. |
| |
Keywords: | |
本文献已被 PubMed 等数据库收录! |
|