| 初生及成年牛眼小梁网,睫状体及视网膜中原癌基因的表达 |
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| 引用本文： | 杨新光,李美玉.初生及成年牛眼小梁网,睫状体及视网膜中原癌基因的表达[J].第四军医大学学报,1997,18(2):136-138. |
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| 作者姓名： | 杨新光 李美玉 |
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| 作者单位： | [1]第四军医大学唐都医院眼科 [2]北京医科大学北大医院眼科 |
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| 摘 要： | 目的：研究正常成年及新生牛眼组织原癌基因的表达水平。方法：分离牛眼组织，提取ＲＮＡ，应用缝隙印迹杂交和Ｎｏｒｔｈｅｒｎ印迹杂交技术，分析眼组织中Ｎ－ｒａｓ，Ｃ－ｍｙｃ和Ｃ－ｆｏｓ基因的表达水平。结果：Ｎ－ｒａｓ基因在牛眼小梁网、睫状体及视网膜神经层均有表达，初生及成年牛间无差异，Ｃ－ｍｙｃ基因在这三种组织中有较高表达，且初生牛比成年牛眼表达水平高。Ｃ－ｆｏｓ基因不表达。结论：正常眼组织中原癌基因的
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| 关 键 词： | 眼 小梁网 睫状体 视网膜 原癌基因 初生牛 表达 |
Expression of proto gene of trabecular meshwork, ciliary body and retina in newborn and adult bovine eye |
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| Yang Xinguang ,Li Meiyu.Expression of proto gene of trabecular meshwork, ciliary body and retina in newborn and adult bovine eye[J].Journal of the Fourth Military Medical University,1997,18(2):136-138. |
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| Authors: | Yang Xinguang Li Meiyu |
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| Institution: | Yang Xinguang 1,Li Meiyu 2 1Department of Ophthalmology,Tangdu Hospital,Fourth Military Medical University,Xi'an 710038 2Bei Da Hospital,Beijing Medical University |
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| Abstract: | Objective: To investigate the expression of proto gene in normal ocular tissue of newborn and adult bovine eyes. Methods: Total RNA were isolated from ocular tissue by Guanidine isothiocyanate method. The expression of N ras . C myc and C fos was analyzed by Northern blot and slot. Results: The expression of C myc in trabecular meshwork, ciliary body and inner layer of retina of natal bovine eye was higher than those in adult bovine eyes. The expression of N ras in those tissues between newborn and adult bovine eye showed the same level. C fos in these tissues was not expressed. Conclusion: The experimental results demonstrated that the expression of proto gene in normal ocular tissues might be relative to the growth and differentiation of ocular cells. |
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| Keywords: | trabecular meshwork ciliary body retina proto gene |
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