| 表达白细胞介素10的系膜细胞基因转移载体的建立 |
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| 引用本文： | 纪玉莲,杨彦强,陈永雄. 表达白细胞介素10的系膜细胞基因转移载体的建立[J]. 中华肾脏病杂志, 1999, 0(1): 17-20 |
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| 作者姓名： | 纪玉莲 杨彦强 陈永雄 |
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| 作者单位： | 中山医科大学附属第一医院肾内科 |
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| 基金项目： | 广东省科委博士点启动基金,中山医科大学校重点基金 |
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| 摘 要： | 目的为特异性地将病毒性白细胞介素１０（ｖＩＬ１０）基因转移到肾脏提供一种手段。方法应用逆转录病毒载体介导的基因转移技术将外源性基因ｖＩＬ１０转移到大鼠肾脏系膜细胞，应用ＲＴＰＣＲ、ＥＬＩＳＡ方法检测其表达，并通过对混合淋巴细胞反应（ＭＬＲ）的影响对其活性进行分析。结果ｖＩＬ１０在转染的系膜细胞表达２００００ｐｇ·（１０６细胞）－１／２４ｈ，应用３ＨＴｄＲ掺入法检测ｖＩＬ１０对ＭＬＲ的影响，实验组明显低于对照组（Ｐ＜００５）。结论外源性基因在系膜细胞有效地转录和表达，并对ＭＬＲ有抑制作用。
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| 关 键 词： | 白细胞介素10 系膜细胞 基因转移 |
Establishment of mesangial cell gene transfer vector expressing interleukin10 |
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| JI Yunian,YANG Yanqiang,CHEN Yongxiong,et al.. Establishment of mesangial cell gene transfer vector expressing interleukin10[J]. Chinese Journal of Nephrology, 1999, 0(1): 17-20 |
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| Authors: | JI Yunian YANG Yanqiang CHEN Yongxiong et al. |
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| Affiliation: | JI Yunian,YANG Yanqiang,CHEN Yongxiong,et al.Department of Nephrology,First Affiliated Hospital,Sun Yatsen University of Medical Sciences,Guangzhou 510080. |
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| Abstract: | Objective To provide a method for specificity transfer vIL10 gene to kidney. Methods vIL10 gene was tranferred into rat glomerular mesangial cells by retroviralmediated gene transfer techniques.The expression of vIL10 was detected by RTPCR,ELISA and its bioactivity was determined by mix lymphocyte reaction (MLR). Results The amount of expression of vIL10 in mesangial cell at least was 20 000 pg(106 cell)-1/24h,and the 3 Hthymidine incorporation in MLR was significantly lower than that in control group (P<005).HZConclusion vIL10 gene can be expressed effectively in mesangial vector,and can inhibit the MLR. |
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| Keywords: | Interleukin10Mesangial cellGene transfer |
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